Linked Life Data

16449971

Source:http://linkedlifedata.com/resource/pubmed/id/16449971

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
2006-6-15
pubmed:abstractText
Macrophage migration inhibitory factor (MIF) has been defined as a novel oncogene. Our previous results have shown that MIF may contribute to the progression of neuroblastoma by (a) inducing N-Myc expression and (b) upregulating the expression of angiogenic factors. The aim of this study was to test whether tumor growth could be inhibited by reduction of endogenous MIF expression in neuroblastoma and clarify the molecular mechanisms underlying MIF reduction on the control of neuroblastoma growth. We established human neuroblastoma cell lines stably expressing antisense MIF (AS-MIF) cDNA. These stable transfectants were characterized by cell proliferation, gene expression profile, tumorigenicity and metastasis in vitro and in vivo. Decreased MIF expression was observed after transfection with AS-MIF in neuroblastoma cells and downregulation of MIF expression significantly correlated with decreased expression of N-Myc, Ras, c-Met and TrkB at protein level. Affymetrix microarray analysis revealed that expression of IL-8 and c-met was inhibited and neuroblastoma-favorable genes such as EPHB6 and BLU were upregulated in MIF reduced cells. Neuroblastoma cell growth exhibited a nearly 80% reduction in AS-MIF transfectants in vitro. Furthermore, mice in which tumors formed after subcutaneous injection of AS-MIF transfectants showed a 90% reduction in tumor growth compared to control. Metastasis in mice was also suppressed dramatically. Our data demonstrate that targeting MIF expression is a promising therapeutic strategy in human neuroblastoma therapy, and also identifies the MIF target genes for further study.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3501-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16449971-Animals, pubmed-meshheading:16449971-Apoptosis, pubmed-meshheading:16449971-Blotting, Western, pubmed-meshheading:16449971-Brain Neoplasms, pubmed-meshheading:16449971-Cell Line, Tumor, pubmed-meshheading:16449971-Cell Proliferation, pubmed-meshheading:16449971-DNA, Antisense, pubmed-meshheading:16449971-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16449971-Gene Expression, pubmed-meshheading:16449971-Humans, pubmed-meshheading:16449971-Macrophage Migration-Inhibitory Factors, pubmed-meshheading:16449971-Mice, pubmed-meshheading:16449971-Neoplasm Metastasis, pubmed-meshheading:16449971-Neoplasms, Experimental, pubmed-meshheading:16449971-Neovascularization, Pathologic, pubmed-meshheading:16449971-Neuroblastoma, pubmed-meshheading:16449971-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16449971-Proto-Oncogene Proteins c-met, pubmed-meshheading:16449971-Proto-Oncogene Proteins c-myc, pubmed-meshheading:16449971-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:16449971-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16449971-Transfection
pubmed:year
2006
pubmed:articleTitle
Inhibition of tumor growth and metastasis in vitro and in vivo by targeting macrophage migration inhibitory factor in human neuroblastoma.
pubmed:affiliation
Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China. reny@ccf.org
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural