Source:http://linkedlifedata.com/resource/pubmed/id/16443778
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rdf:type | |
lifeskim:mentions |
umls-concept:C0015101,
umls-concept:C0017337,
umls-concept:C0022131,
umls-concept:C0030705,
umls-concept:C0033684,
umls-concept:C0185023,
umls-concept:C0221099,
umls-concept:C0237497,
umls-concept:C0332307,
umls-concept:C1171362,
umls-concept:C1314972,
umls-concept:C1523873,
umls-concept:C1749467,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781
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pubmed:issue |
2
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pubmed:dateCreated |
2006-1-30
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pubmed:abstractText |
Exocytosis of insulin is dependent on the soluble N-ethylmaleimide attachment protein receptor (SNARE) complex proteins in the B-cells. We assessed insulin release as well as gene and protein expression of SNARE complex protein in isolated pancreatic islets of type 2 diabetic patients (n = 4) and nondiabetic control subjects (n = 4). In islets from the diabetic patients, insulin responses to 8.3 and 16.7 mmol/l glucose were markedly reduced compared with control islets (4.7 +/- 0.3 and 8.4 +/- 1.8 vs. 17.5 +/- 0.1 and 24.3 +/- 1.2 microU . islet(-1) . h(-1), respectively; P < 0.001). Western blot analysis revealed decreased amounts of islet SNARE complex and SNARE-modulating proteins in diabetes: syntaxin-1A (21 +/- 5% of control levels), SNAP-25 (12 +/- 4%), VAMP-2 (7 +/- 4%), nSec1 (Munc 18; 34 +/- 13%), Munc 13-1 (27 +/- 4%), and synaptophysin (64 +/- 7%). Microarray gene chip analysis, confirmed by quantitative PCR, showed that gene expression was decreased in diabetes islets: syntaxin-1A (27 +/- 2% of control levels), SNAP-25 (31 +/- 7%), VAMP-2 (18 +/- 3%), nSec1 (27 +/- 5%), synaptotagmin V (24 +/- 2%), and synaptophysin (12 +/- 2%). In conclusion, these data support the view that decreased islet RNA and protein expression of SNARE and SNARE-modulating proteins plays a role in impaired insulin secretion in type 2 diabetic patients. It remains unclear, however, to which extent this defect is primary or secondary to, e.g., glucotoxicity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0012-1797
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
435-40
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16443778-Aged,
pubmed-meshheading:16443778-Aged, 80 and over,
pubmed-meshheading:16443778-Diabetes Mellitus, Type 2,
pubmed-meshheading:16443778-Female,
pubmed-meshheading:16443778-Gene Expression Profiling,
pubmed-meshheading:16443778-Gene Expression Regulation,
pubmed-meshheading:16443778-Glucagon,
pubmed-meshheading:16443778-Humans,
pubmed-meshheading:16443778-Insulin,
pubmed-meshheading:16443778-Islets of Langerhans,
pubmed-meshheading:16443778-Male,
pubmed-meshheading:16443778-Middle Aged,
pubmed-meshheading:16443778-SNARE Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Impaired gene and protein expression of exocytotic soluble N-ethylmaleimide attachment protein receptor complex proteins in pancreatic islets of type 2 diabetic patients.
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pubmed:affiliation |
Department of Molecular Medicine and Surgery, Endocrine and Diabetes Unit, Karolinska University Hospital, Stockholm, Sweden. claes-goran.ostenson@karolinska.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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