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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-7-3
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pubmed:abstractText |
TGF-beta1 has been known as an important factor in tubulointerstitial fibrosis which is a common process in most progressive renal diseases. We hypothesized that the interstitial fibrosis could be prevented by abolishing TGF-beta1 function in unilateral ureteral obstruction (UUO)-induced renal fibrosis. shRNA vectors were generated to suppress TGF-beta1 expression at a high glucose concentration which allowed the maximal induction of TGF-beta1 in primary rat mesangial cells. An shRNA vector, designated shTB1d, significantly suppressed TGF-beta1 in both transcriptional and translational levels in vitro cultured cells and in vivo fibrosis-induced mouse kidney, accompanied by the suppression of target genes (e.g., type I collagen and PAI-1) of TGF-beta1. Furthermore, the shTB1d suppressed the expression of TGF-beta1 and type I collagen in tubulointerstitial cells until day 7 after UUO-induced fibrosis, but none- or vector-treated mice maintained their expression, suggesting that the TGF-beta1 shRNA delays the process of renal fibrosis in UUO mouse model. This work would provide a valuable tool to prevent tubulointerstitial fibrosis using RNA interference strategy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-4800
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pubmed:author |
pubmed-author:ChaeYoung-MiYM,
pubmed-author:ChangYoung-ChaeYC,
pubmed-author:HwangMeeyulM,
pubmed-author:JeonJae-PilJP,
pubmed-author:KimHye-JinHJ,
pubmed-author:KimKyung-HyunKH,
pubmed-author:LeeTae-SungTS,
pubmed-author:LeeYun-SikYS,
pubmed-author:NohHey-JeongHJ,
pubmed-author:OhHoon-KyuHK,
pubmed-author:ParkKwan-KyuKK
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16443218-Animals,
pubmed-meshheading:16443218-Fibrosis,
pubmed-meshheading:16443218-Gene Therapy,
pubmed-meshheading:16443218-Genetic Vectors,
pubmed-meshheading:16443218-Glomerular Mesangium,
pubmed-meshheading:16443218-Glucose,
pubmed-meshheading:16443218-Kidney Diseases,
pubmed-meshheading:16443218-Kidney Tubules,
pubmed-meshheading:16443218-Mice,
pubmed-meshheading:16443218-RNA, Small Interfering,
pubmed-meshheading:16443218-RNA Interference,
pubmed-meshheading:16443218-Rats,
pubmed-meshheading:16443218-Transforming Growth Factor beta,
pubmed-meshheading:16443218-Transforming Growth Factor beta1,
pubmed-meshheading:16443218-Ureteral Obstruction
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pubmed:year |
2006
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pubmed:articleTitle |
TGF-beta1 siRNA suppresses the tubulointerstitial fibrosis in the kidney of ureteral obstruction.
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pubmed:affiliation |
Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Republic of Korea.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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