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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-1-30
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pubmed:abstractText |
The formation of N-formylkynurenine by dioxygenation of tryptophan was detected in peptides from rice leaf and potato tuber mitochondria. Proteins in matrix and membrane fractions were separated by two-dimensional gel electrophoresis and identified using a Q-TOF mass spectrometer. N-Formylkynurenine was detected in 29 peptides representing 17 different proteins. With one exception, the oxidation-sensitive aconitase, all of these proteins were either redox active themselves or subunits in redox-active enzyme complexes. The same site was modified in (i) several adjacent spots containing the P protein of the glycine decarboxylase complex, (ii) two different isoforms of the mitochondrial processing peptidase in complex III, and (iii) the same tryptophan residues in Mn-superoxide dismutase in both rice and potato mitochondria. This indicates that Trp oxidation is a selective process.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0891-5849
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
430-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16443157-Aconitate Hydratase,
pubmed-meshheading:16443157-Amino Acid Sequence,
pubmed-meshheading:16443157-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:16443157-Kynurenine,
pubmed-meshheading:16443157-Mitochondria,
pubmed-meshheading:16443157-Mitochondrial Proteins,
pubmed-meshheading:16443157-Molecular Sequence Data,
pubmed-meshheading:16443157-Oxidation-Reduction,
pubmed-meshheading:16443157-Plant Leaves,
pubmed-meshheading:16443157-Plant Proteins,
pubmed-meshheading:16443157-Sequence Homology, Amino Acid,
pubmed-meshheading:16443157-Solanum tuberosum,
pubmed-meshheading:16443157-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16443157-Superoxide Dismutase,
pubmed-meshheading:16443157-Tryptophan
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pubmed:year |
2006
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pubmed:articleTitle |
Protein oxidation in plant mitochondria detected as oxidized tryptophan.
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pubmed:affiliation |
Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. imm@kvl.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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