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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-9-8
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pubmed:abstractText |
Two new vectors containing the xylE gene (encoding catechol-2,3-dioxygenase) of Pseudomonas putida were constructed that serve as the source of the xylE cassette. These vectors are based on the kanamycin-resistance-encoding plasmid, pKAN18. The promoter-less xylE gene is flanked by several restriction enzyme sites that allow for easy excision of this gene in the form of a cassette containing a ribosome-binding site, 7 bp upstream from the start codon. These cassettes lack any transcriptional termination signals downstream from the stop codon.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
117
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pubmed:geneSymbol |
xylE
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1644310-Base Sequence,
pubmed-meshheading:1644310-Catechol 2,3-Dioxygenase,
pubmed-meshheading:1644310-Cloning, Molecular,
pubmed-meshheading:1644310-DNA, Bacterial,
pubmed-meshheading:1644310-Dioxygenases,
pubmed-meshheading:1644310-Molecular Sequence Data,
pubmed-meshheading:1644310-Oxygenases,
pubmed-meshheading:1644310-Plasmids,
pubmed-meshheading:1644310-Pseudomonas putida,
pubmed-meshheading:1644310-Restriction Mapping
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pubmed:year |
1992
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pubmed:articleTitle |
Plasmids with easily excisable xylE cassettes.
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pubmed:affiliation |
Department of Microbiology, University of Maryland, College Park 20742.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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