Source:http://linkedlifedata.com/resource/pubmed/id/16442735
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2006-3-13
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pubmed:abstractText |
Recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Babesia bigemina infection by using a full-length B. bigemina rhoptry-associated protein 1 (rRAP-1) and the truncated C-terminal RAP-1 (rRAP-1/CT). While the rRAP-1 showed cross reactivity between B. bigemina- and Babesia bovis-infected bovine sera, the rRAP-1/CT was highly specific to B. bigemina-infected bovine sera and proved useful in the detection of sequential sera collected from an experimentally infected cow during the acute and latent infection. The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0304-4017
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
28-35
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16442735-Animals,
pubmed-meshheading:16442735-Antigens, Protozoan,
pubmed-meshheading:16442735-Babesia,
pubmed-meshheading:16442735-Babesiosis,
pubmed-meshheading:16442735-Cattle,
pubmed-meshheading:16442735-Cattle Diseases,
pubmed-meshheading:16442735-Cross Reactions,
pubmed-meshheading:16442735-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16442735-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:16442735-Gene Expression,
pubmed-meshheading:16442735-Mice,
pubmed-meshheading:16442735-Protozoan Proteins,
pubmed-meshheading:16442735-Recombinant Proteins,
pubmed-meshheading:16442735-Sensitivity and Specificity
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pubmed:year |
2006
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pubmed:articleTitle |
Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay.
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pubmed:affiliation |
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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