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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-9-9
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pubmed:abstractText |
A new type of mitogenic factor (protein) was purified from the culture supernatant of a strain of Streptococcus pyogenes by SP-Sephadex C-25 column chromatography, preparative isoelectric focusing and reversed-phase high-performance liquid chromatography. The purified factor, showing marked mitogenic activity in rabbit peripheral blood lymphocytes, gave a single-band staining for protein on SDS-PAGE. The molecular weight of the purified mitogenic factor was determined to be 25,370, which was different from those calculated from reported amino acid sequences deduced from 4 different nucleotide sequences of 3 kinds of streptococcal pyrogenic exotoxins (two SPEAs, SPEB and SPEC). The amino acid sequence of the N-terminal region of the purified mitogenic factor was determined to be Gln-Thr-Gln-Val-Ser-Asn-Asp-Val-Val-Leu-Asn-Asp-Gly-Ala-Ser-Lys-Tyr-Leu- Asn-Glu - Ala-, which was also different from the reported N-terminal sequences deduced from the 4 different nucleotide sequences. These data indicate that this mitogenic factor is distinct from the already described streptococcal pyrogenic exotoxins.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
308
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1644200-Amino Acid Sequence,
pubmed-meshheading:1644200-Amino Acids,
pubmed-meshheading:1644200-Chromatography, High Pressure Liquid,
pubmed-meshheading:1644200-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1644200-Isoelectric Focusing,
pubmed-meshheading:1644200-Mass Spectrometry,
pubmed-meshheading:1644200-Mitogens,
pubmed-meshheading:1644200-Molecular Sequence Data,
pubmed-meshheading:1644200-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1644200-Streptococcus pyogenes
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pubmed:year |
1992
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pubmed:articleTitle |
A new type of mitogenic factor produced by Streptococcus pyogenes.
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pubmed:affiliation |
Shionogi Institute for Medical Science, Osaka, Japan.
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pubmed:publicationType |
Journal Article
|