Linked Life Data

16440313

Source:http://linkedlifedata.com/resource/pubmed/id/16440313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-5-3
pubmed:abstractText
Throughout oogenesis, huge amounts of RNA are produced that are needed for early development. Early stages of oocyte development are characterized by high transcriptional activity whereas translation of maternal RNA dominates late stages. Nuclear pore complexes (NPCs), located in the nuclear envelope (NE), mediate bidirectional macromolecule exchange between the nuclear and cytosolic compartments including RNA export. Here, we report on structural correlates of this transport pathway at single NPC level. Using atomic force microscopy (AFM), we imaged the nucleoplasmic ("inner") surface of the NE of Xenopus laevis oocytes in different stages of development. We found that NPC frequency per nucleus increases with maturation. However, individual NPCs are more active in immature stages. In early stages, known for high transcriptional activity, we found nearly 10% of NPC central channels plugged with a 400-800 kDa mass. In contrast, the incidence of plugged NPCs was below 1% in late oocyte stages. On-site RNA digestion led to a change in plug shape from prominent to flat while plug mass decreased by almost 20%. Quantitative AFM analysis revealed that RNase exposure reduced total nucleoplasmic NPC mass by about 58 and 25% in early and late stage oocytes, respectively. We conclude: (i) NPCs of immature oocytes are more active in RNA transport, (ii) Plugs identified at the nucleoplasmic entrance of NPC central channels represent ribonucleoproteins exiting the nucleus, (iii) RNA is a structural component of the NPC nanomachine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0730-2312
pubmed:author
pubmed:copyrightInfo
(c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
567-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Plugs in nuclear pores: transcripts in early oocyte development identified with nanotechniques.
pubmed:affiliation
Institute of Physiology II, University of Münster, Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't