|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
2006-2-1
|
|
pubmed:abstractText |
To understand the role of RNA-binding proteins (RBPs) in the regulation of gene expression, methods are needed for the in vivo identification of RNA-protein interactions. We have developed the peptide nucleic acid (PNA)-assisted identification of RBP technology to enable the identification of proteins that complex with a target RNA in vivo. Specific regions of the 3' and 5' UTRs of ankylosis mRNA were targeted by antisense PNAs transported into cortical neurons by the cell-penetrating peptide transportan 10. An array of proteins was isolated in complex with or near the targeted regions of the ankylosis mRNA through UV-induced crosslinking of the annealed PNA-RNA-RBP complex. The first evidence for pharmacological modulation of these specific protein-RNA associations was observed. These data show that the PNA-assisted identification of the RBP technique is a reliable method to rapidly identify proteins interacting in vivo with the target RNA.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10421639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10567417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10700145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10712515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10799534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10834926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10894769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-10930519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11027353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11074006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11121017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11167067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11207359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11249622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11255003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11283311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11283721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11564879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11718666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11719188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-11886857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12097415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12110596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12440850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12462405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12570854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12575950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12871776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-12957960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-14500847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-14523087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-14615540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-1508690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-15122260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-1962210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-1993689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-2049599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-3881256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-6731838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-7692304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-8102206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-8602363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-8826475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-8987809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9104594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9281585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9391005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9438412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9743120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432185-9845364
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Nucleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
31
|
|
pubmed:volume |
103
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1557-62
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
