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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-2-1
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pubmed:abstractText |
Genetically primed adult C57BL mice were deleted of exon 5 of the gene encoding the transcription factor PU.1 by IFN activation of Cre recombinase. After a 13-week delay, conditionally deleted (PU.1(-/-)) mice began dying of myeloid leukemia, and 95% of the mice surviving from early postinduction death developed transplantable myeloid leukemia whose cells were deleted of PU.1 and uniformly Gr-1 positive. The leukemic cells formed autonomous colonies in semisolid culture with varying clonal efficiency, but colony formation was enhanced by IL-3 and sometimes by granulocyte-macrophage colony-stimulating factor. Nine of 13 tumors analyzed had developed a capacity for autocrine IL-3 or granulocyte-macrophage colony-stimulating factor production, and there was evidence of rearrangement of the IL-3 gene. Acquisition of autocrine growth-factor production and autonomous growth appeared to be major events in the transformation of conditionally deleted PU.1(-/-) cells to fully developed myeloid leukemic populations.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-10203717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-10438716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-11157490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-11916518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-12130514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-12453885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-12563308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-14597609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-14630084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-14739214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15146183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15304397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15657291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15867096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15914556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-15914558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-2108861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-3878229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-4513522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-7544853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-8079170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-8896458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-9133423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-9324276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-9573007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16432184-9716585
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1486-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16432184-Animals,
pubmed-meshheading:16432184-Bone Marrow Cells,
pubmed-meshheading:16432184-Cell Line, Tumor,
pubmed-meshheading:16432184-Cell Transplantation,
pubmed-meshheading:16432184-Flow Cytometry,
pubmed-meshheading:16432184-Gene Deletion,
pubmed-meshheading:16432184-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:16432184-Growth Substances,
pubmed-meshheading:16432184-Interleukin-3,
pubmed-meshheading:16432184-Leukemia,
pubmed-meshheading:16432184-Leukemia, Myeloid,
pubmed-meshheading:16432184-Mice,
pubmed-meshheading:16432184-Mice, Inbred C57BL,
pubmed-meshheading:16432184-Mice, Transgenic,
pubmed-meshheading:16432184-Neoplasm Transplantation,
pubmed-meshheading:16432184-Proto-Oncogene Proteins,
pubmed-meshheading:16432184-Spleen,
pubmed-meshheading:16432184-Time Factors,
pubmed-meshheading:16432184-Trans-Activators
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pubmed:year |
2006
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pubmed:articleTitle |
Inactivation of PU.1 in adult mice leads to the development of myeloid leukemia.
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pubmed:affiliation |
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Victoria, Australia. metcalf@wehi.edu.au
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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