Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-2-14
pubmed:abstractText
Extracellular ATP might act as a trophic factor on growing axons during development of the CNS via P2 receptors. In the present study the postnatal presence of selected P2 receptor subtypes was analyzed and their putative trophic capacity in entorhino-hippocampal slice co-cultures of mouse brain was tested. The effect of the P2 receptor ligands 2-methylthioadenosine-5'-triphosphate (P2X/Y receptor agonist) and pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (P2X/Y receptor antagonist) on axonal growth and fiber density of biocytin-labeled hippocampal projections was compared both with untreated cultures and with cultures treated with artificial cerebrospinal fluid. After 10 days in vitro, double immunofluorescence labeling revealed the expression of P2X(1), P2X(2), P2X(4) as well as P2Y(1) and P2Y(2) receptors in the examined regions of entorhinal fiber termination. Further, quantitative analysis of identified biocytin-traced entorhinal fibers showed a significant increase in fiber density in the dentate gyrus after incubation of the slices with the P2 receptor agonist 2-methylthioadenosine-5'-triphosphate. This neurite outgrowth promoting effect was completely abolished by the P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid. Our in vitro data indicate that ATP via its P2X and P2Y receptors can shape hippocampal connectivity during development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-11
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16431028-Adenosine Triphosphate, pubmed-meshheading:16431028-Animals, pubmed-meshheading:16431028-Axons, pubmed-meshheading:16431028-Cell Count, pubmed-meshheading:16431028-Coculture Techniques, pubmed-meshheading:16431028-Dentate Gyrus, pubmed-meshheading:16431028-Entorhinal Cortex, pubmed-meshheading:16431028-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:16431028-Hippocampus, pubmed-meshheading:16431028-Immunohistochemistry, pubmed-meshheading:16431028-Mice, pubmed-meshheading:16431028-Mice, Inbred C57BL, pubmed-meshheading:16431028-Microscopy, Confocal, pubmed-meshheading:16431028-Nerve Fibers, pubmed-meshheading:16431028-Organ Culture Techniques, pubmed-meshheading:16431028-Purinergic P2 Receptor Agonists, pubmed-meshheading:16431028-Pyridoxal Phosphate, pubmed-meshheading:16431028-Stimulation, Chemical, pubmed-meshheading:16431028-Thionucleotides
pubmed:year
2006
pubmed:articleTitle
P2 receptor-stimulation influences axonal outgrowth in the developing hippocampus in vitro.
pubmed:affiliation
Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Haertelstrasse 16-18, D-04107 Leipzig, Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't