Source:http://linkedlifedata.com/resource/pubmed/id/16430985
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2006-4-24
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| pubmed:databankReference | |
| pubmed:abstractText |
The healthy skin of a seven-year-old female goat (Capra hircus) was sampled with a cotton tip swab. Total genomic DNA was extracted from the sample and subjected to multiply primed rolling-circle amplification (RCA). Restriction analysis showed that the complete genome of a papillomavirus was amplified, measuring approximately 7,600 bp in length. The Capra hircus papillomavirus (ChPV-1) genome was cloned from this rolling-circle amplification product, and the complete nucleotide sequence was determined. The ChPV-1 genome counts 7,542 bp, and contains the typical papillomaviral open reading frames (ORFs). Based on a concatenated alignment of the E1, E2, L1 and L2 open reading frames of ChPV-1 and 54 other animal and human papillomavirus types, a neighbor-joining phylogenetic tree was constructed. In this tree ChPV-1 clusters with BPV-3, -4 and -6. Pairwise nucleotide sequence alignments of the L1 open reading frame of ChPV-1 with its closest relatives showed less than 60% similarity, placing the ChPV-1 in a novel genus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0168-1702
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
118
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
164-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16430985-Animals,
pubmed-meshheading:16430985-Base Sequence,
pubmed-meshheading:16430985-Cloning, Molecular,
pubmed-meshheading:16430985-DNA, Viral,
pubmed-meshheading:16430985-Female,
pubmed-meshheading:16430985-Genome, Viral,
pubmed-meshheading:16430985-Goats,
pubmed-meshheading:16430985-Molecular Sequence Data,
pubmed-meshheading:16430985-Nucleic Acid Amplification Techniques,
pubmed-meshheading:16430985-Open Reading Frames,
pubmed-meshheading:16430985-Papillomaviridae,
pubmed-meshheading:16430985-Papillomavirus Infections,
pubmed-meshheading:16430985-Phylogeny,
pubmed-meshheading:16430985-Restriction Mapping,
pubmed-meshheading:16430985-Sequence Analysis, DNA,
pubmed-meshheading:16430985-Sequence Homology, Amino Acid,
pubmed-meshheading:16430985-Sequence Homology, Nucleic Acid,
pubmed-meshheading:16430985-Skin,
pubmed-meshheading:16430985-Viral Proteins
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Genetic characterization of the Capra hircus papillomavirus: a novel close-to-root artiodactyl papillomavirus.
|
| pubmed:affiliation |
Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|