16430139

Source:http://linkedlifedata.com/resource/pubmed/id/16430139

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0001674, umls-concept:C0001721, umls-concept:C0026046, umls-concept:C0205148, umls-concept:C1706211, umls-concept:C1707798, umls-concept:C1880352
pubmed:issue	12
pubmed:dateCreated	2006-1-24
pubmed:abstractText	The dynamics of disassembly of microtubules deposited on surfaces is shown to be strongly dependent on the electrostatic interaction between the microtubule and the substrate. Fluorescence microscopy of microtubules adsorbed on a Poly-L-Lysine film and immersed in pure water show a drastic decrease in disassembly velocity compared to the microtubules in bulk water solutions. While microtubules suspended in pure water disassemble in seconds, the dissociation velocity of microtubules adsorbed on a Poly-L-Lysine film ranges from 0.8 to 1.0 microm/min in pure water. Kinetic Monte Carlo simulations of the microtubule dynamics indicate that a decrease in the dissociation velocity of unstable microtubules can be achieved by reducing the heterodimer dissociation rate constant of tubulin heterodimers constituting a single protofilament, adsorbed to the Poly-L-Lysine film. This model suggests that the reduction of the dissociation velocity originates from the electrostatic interactions between the positively charged amino groups of the Poly-L-Lysine film and the negatively charged microtubule surface.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101088195
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Dec
pubmed:issn	1533-4880
pubmed:author	pubmed-author:DeymierP APA, pubmed-author:GuzmanRR, pubmed-author:HoyingJJ, pubmed-author:PalusinskiOO, pubmed-author:RaghavanSS, pubmed-author:UmnovMM, pubmed-author:YangYiY, pubmed-author:ZelinskiB J JBJ
pubmed:issnType	Print
pubmed:volume	5
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	2050-6
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:16430139-Adsorption, pubmed-meshheading:16430139-Computer Simulation, pubmed-meshheading:16430139-Microtubules, pubmed-meshheading:16430139-Nanotechnology, pubmed-meshheading:16430139-Surface Properties
pubmed:year	2005
pubmed:articleTitle	Adsorption of a microtubule on a charged surface affects its disassembly dynamics.
pubmed:affiliation	Department of Materials Science and Engineering, Nano-Biomolecular Engineering Science and Technology (n-BEST) Program, The University of Arizona, Tucson AZ 85721, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't