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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7084
pubmed:dateCreated
2006-3-30
pubmed:abstractText
Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set enabled a de novo characterization of the composition and organization of the cellular machinery. The ensemble of cellular proteins partitions into 491 complexes, of which 257 are novel, that differentially combine with additional attachment proteins or protein modules to enable a diversification of potential functions. Support for this modular organization of the proteome comes from integration with available data on expression, localization, function, evolutionary conservation, protein structure and binary interactions. This study provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1476-4687
pubmed:author
pubmed-author:AloyPatrickP, pubmed-author:BastuckSonjaS, pubmed-author:BauerAndreasA, pubmed-author:BoescheMarkusM, pubmed-author:BorkPeerP, pubmed-author:BouwmeesterTewisT, pubmed-author:CasariGeorgG, pubmed-author:DümpelfeldBirgitB, pubmed-author:DrewesGerardG, pubmed-author:EdelmannAngelaA, pubmed-author:GavinAnne-ClaudeAC, pubmed-author:GrandiPaolaP, pubmed-author:HeurtierMarie-AnneMA, pubmed-author:HoefertChristianC, pubmed-author:HoffmanVerenaV, pubmed-author:HooperSeanS, pubmed-author:HudakManuelaM, pubmed-author:JensenLars JuhlLJ, pubmed-author:KleinKarinK, pubmed-author:KrauseRolandR, pubmed-author:KusterBernhardB, pubmed-author:MarziochMartinaM, pubmed-author:MichonAnne-MarieAM, pubmed-author:NeubauerGitteG, pubmed-author:RauChristinaC, pubmed-author:RemorMaritaM, pubmed-author:RickJens MJM, pubmed-author:RudiTatjanaT, pubmed-author:RussellRobert BRB, pubmed-author:SchelderMalgorzataM, pubmed-author:SchirleMarkusM, pubmed-author:Superti-FurgaGiulioG
pubmed:issnType
Electronic
pubmed:day
30
pubmed:volume
440
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
631-6
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Proteome survey reveals modularity of the yeast cell machinery.
pubmed:affiliation
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
pubmed:publicationType
Journal Article