Linked Life Data

16428807

Source:http://linkedlifedata.com/resource/pubmed/id/16428807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2006-3-27
pubmed:abstractText
The fission yeast HIRA proteins Hip1 and Slm9 are members of an evolutionarily conserved family of histone chaperones that are implicated in nucleosome assembly. Here we have used single-step affinity purification and mass spectrometry to identify factors that interact with both Hip1 and Slm9. This analysis identified Hip3, a previously uncharacterized 187-kDa protein, with similarity to S. cerevisiae Hir3. Consistent with this, cells disrupted for hip3+ exhibit a range of growth defects that are similar to those associated with loss of Hip1 and Slm9. These include temperature sensitivity, a cell cycle delay, and synthetic lethality with cdc25-22. Furthermore, genetic analysis also indicates that disruption of hip3+ is epistatic with mutation of hip1+ and slm9+. Mutation of hip3+ alleviates transcriptional silencing at several heterochromatic loci, including in the outer (otr) centromeric repeats, indicating that Hip3 is required for the integrity of pericentric heterochromatin. As a result, loss of Hip3 function leads to high levels of minichromosome loss and an increased frequency of lagging chromosomes during mitosis. Importantly, the function of Hip1, Slm9, and Hip3 is not restricted to constitutive heterochromatic loci, since these proteins also repress the expression of a number of genes, including the Tf2 retrotransposons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8732-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16428807-Chromatography, Affinity, pubmed-meshheading:16428807-Chromosome Segregation, pubmed-meshheading:16428807-Chromosomes, Fungal, pubmed-meshheading:16428807-Fluorescein-5-isothiocyanate, pubmed-meshheading:16428807-Fluorescent Dyes, pubmed-meshheading:16428807-Gene Expression Regulation, Fungal, pubmed-meshheading:16428807-Gene Silencing, pubmed-meshheading:16428807-Indoles, pubmed-meshheading:16428807-Mass Spectrometry, pubmed-meshheading:16428807-Microscopy, Fluorescence, pubmed-meshheading:16428807-Precipitin Tests, pubmed-meshheading:16428807-RNA, pubmed-meshheading:16428807-Schizosaccharomyces, pubmed-meshheading:16428807-Schizosaccharomyces pombe Proteins, pubmed-meshheading:16428807-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:16428807-Transcription, Genetic
pubmed:year
2006
pubmed:articleTitle
Hip3 interacts with the HIRA proteins Hip1 and Slm9 and is required for transcriptional silencing and accurate chromosome segregation.
pubmed:affiliation
Institute of Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't