Linked Life Data

16427635

Source:http://linkedlifedata.com/resource/pubmed/id/16427635

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-1-31
pubmed:databankReference
pubmed:abstractText
Tight junction (TJ) constitutes the barrier by controlling the passage of ions and molecules via paracellular pathway and the movement of proteins and lipids between apical and basolateral domains of the plasma membrane. Claudins, occludin, and junctional adhesion molecules are the major three transmembrane proteins at TJ. This study focuses a newly identified mammalian TJ gene, claudin-19, in kidneys. Mouse claudin-19 composes of 224 amino acids and shares 98.2% and 95% amino acid homology with rat and human, respectively; the most evolutionary-related claudins are claudin-1 and -7, which share approximately 75% DNA sequence homology with claudin-19. Claudin-19 is abundantly expressed in the mouse and rat kidneys among the organs examined by Northern blots, and to a much less extent, also found in brain by RT-PCR. Claudin-19 and zonula occludens-1 (ZO-1) are localized at junctional regions of Madin-Darby canine kidney (MDCK) cells by immunofluorescent microscopy. In addition, ZO-1 is found in the claudin-19-associated protein complexes in MDCK cells by co-immunoprecipitation. Using aquaporin-1 and aquaporin-2 antibodies as markers for different renal segment, strong expression of claudin-19 was observed in distal tubules of the cortex as well as in the collecting ducts of the medulla. To less extent, claudin-19 is also present in the proximal tubules (cortex) and in the loop of Henle (medulla). Furthermore, intense claudin-19 immunoreactivity is found co-localized with the ZO-1 in kidneys from postnatal day 15, day 45, and adult rats and mice. Similar localizations of claudin-19 and ZO-1 are also observed in human kidneys. Since these renal segments are mainly for controlling the paracellular cation transport, it is suggested that claudin-19 may participate in these processes. In human polycystic kidneys, decreased expression and dyslocalization of claudin-19 are noticed, suggesting a possible correlation between claudin-19 and renal disorders. Taken together, claudin-19 is a claudin isoform that is highly and specifically expressed in renal tubules with a putative role in TJ homeostasis in renal physiology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
580
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
923-31
pubmed:dateRevised
2007-6-14
pubmed:meshHeading
pubmed-meshheading:16427635-Animals, pubmed-meshheading:16427635-Aquaporin 1, pubmed-meshheading:16427635-Aquaporin 2, pubmed-meshheading:16427635-Base Sequence, pubmed-meshheading:16427635-Cell Line, pubmed-meshheading:16427635-Dogs, pubmed-meshheading:16427635-Gene Expression Regulation, pubmed-meshheading:16427635-Humans, pubmed-meshheading:16427635-Ion Transport, pubmed-meshheading:16427635-Kidney Tubules, Collecting, pubmed-meshheading:16427635-Kidney Tubules, Distal, pubmed-meshheading:16427635-Loop of Henle, pubmed-meshheading:16427635-Membrane Proteins, pubmed-meshheading:16427635-Molecular Sequence Data, pubmed-meshheading:16427635-Organ Specificity, pubmed-meshheading:16427635-Phosphoproteins, pubmed-meshheading:16427635-Polycystic Kidney Diseases, pubmed-meshheading:16427635-Rats, pubmed-meshheading:16427635-Rats, Sprague-Dawley, pubmed-meshheading:16427635-Tight Junctions
pubmed:year
2006
pubmed:articleTitle
Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease.
pubmed:affiliation
Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong, SAR, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't