Source:http://linkedlifedata.com/resource/pubmed/id/16424058
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2006-1-20
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pubmed:abstractText |
The activity of growth factors is crucial for tumor progression. We previously characterized a secreted fibroblast growth factor-binding protein (FGF-BP1) as a chaperone molecule, which enhances the biological functions of FGFs by releasing FGFs from the extracellular matrix. Here, we characterize the frequency and pattern of FGF-BP1 expression during the malignant progression of pancreas and colorectal carcinoma. For this, we generated monoclonal antibodies that detect FGF-BP1 protein in formalin-fixed, paraffin-embedded tissues and applied in situ hybridization to detect FGF-BP1 mRNA in adjacent tissue sections. FGF-BP1 protein and mRNA were found up-regulated (>70% positive) in parallel (r = 0.70, P < 0.0001) in colon adenoma (n = 9) as well as primary (n = 46) and metastatic (n = 71) colorectal cancers relative to normal colon epithelia (all P < 0.0001, versus normal). Similarly, pancreatitis (n = 17), pancreatic intraepithelial neoplasia (n = 80), and pancreatic adenocarcinoma (n = 67) showed a significant up-regulation of FGF-BP1 compared with normal pancreas (n = 42; all P < 0.0001, relative to normal). Furthermore, the biological activity of FGF-BP1 is neutralized by one of the antibodies, suggesting the potential for antibody-based therapeutic targeting. We propose that the up-regulation of the secreted FGF-BP1 protein during initiation of pancreas and colon neoplasia could make this protein a possible serum marker indicating the presence of high-risk premalignant lesions.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FGFBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pancrelipase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-5472
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1191-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16424058-Adenocarcinoma,
pubmed-meshheading:16424058-Antibodies, Monoclonal,
pubmed-meshheading:16424058-Carrier Proteins,
pubmed-meshheading:16424058-Cell Transformation, Neoplastic,
pubmed-meshheading:16424058-Colonic Neoplasms,
pubmed-meshheading:16424058-Disease Progression,
pubmed-meshheading:16424058-Gene Expression Profiling,
pubmed-meshheading:16424058-Humans,
pubmed-meshheading:16424058-In Situ Hybridization,
pubmed-meshheading:16424058-Pancreatic Neoplasms,
pubmed-meshheading:16424058-Pancreatitis,
pubmed-meshheading:16424058-Pancrelipase,
pubmed-meshheading:16424058-RNA, Messenger,
pubmed-meshheading:16424058-Risk Assessment,
pubmed-meshheading:16424058-Tumor Cells, Cultured,
pubmed-meshheading:16424058-Up-Regulation
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pubmed:year |
2006
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pubmed:articleTitle |
Expression of a fibroblast growth factor-binding protein during the development of adenocarcinoma of the pancreas and colon.
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pubmed:affiliation |
Lombardi Cancer Center, Georgetown University, 3970 Reservoir Road, Washington, DC 20057, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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