Source:http://linkedlifedata.com/resource/pubmed/id/16420439
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2006-1-19
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| pubmed:abstractText |
Radial glial cells are the main component of the embryonic cortical ventricular zone (VZ), producing deep-layer excitatory neurons in the early stage and upper-layer excitatory neurons in the late stage of development. Previous studies have suggested that the laminar fate of deep-layer neurons might be determined by early-stage-specific secretory or transmembrane molecules (S/TMs) in the VZ. However, the different properties required to produce the different types of neurons in early-stage and late-stage VZ cells are largely unknown. Herein, we investigated the stage-dependent transcriptional profiles of the ventricular side of the mouse cortex, which was manually dissected at embryonic day (E)12, E14 and E16, and identified 3985 'VZ-enriched' genes, regulated stage-dependently, by GeneChip analysis. These molecules were classified into nine types based on stage-dependent regulation patterns. Prediction programs for the S/TMs revealed 659 'VZ-enriched' S/TMs. In situ hybridization and real-time PCR analysis for several of these molecules showed results consistent with the statistical analysis of the GeneChip experiments. Moreover, we identified 17 cell cycle-related early-stage and 'VZ-enriched' molecules. These molecules included not only those involved in cell cycle progression, but also essential molecules for DNA double-strand break repair, such as Rad51 and Rpa1. These results suggest that the early stage-VZ cells, which produce both deep- and upper-layer neurons, and the late-stage VZ cells, which produce only upper-layer neurons, are intrinsically different. The gene lists presented here will be useful for the investigation of stage-dependent changes in VZ cells and their regulatory mechanisms in the developing cortex.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0953-816X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
296-308
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16420439-Animals,
pubmed-meshheading:16420439-Blotting, Western,
pubmed-meshheading:16420439-Cerebral Cortex,
pubmed-meshheading:16420439-Cerebral Ventricles,
pubmed-meshheading:16420439-Embryo, Mammalian,
pubmed-meshheading:16420439-Female,
pubmed-meshheading:16420439-Gene Expression Regulation, Developmental,
pubmed-meshheading:16420439-In Situ Hybridization,
pubmed-meshheading:16420439-Male,
pubmed-meshheading:16420439-Mice,
pubmed-meshheading:16420439-Mice, Inbred ICR,
pubmed-meshheading:16420439-Nerve Tissue Proteins,
pubmed-meshheading:16420439-Neurons,
pubmed-meshheading:16420439-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16420439-Pregnancy,
pubmed-meshheading:16420439-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:16420439-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16420439-alpha Catenin
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Identification of ventricular-side-enriched molecules regulated in a stage-dependent manner during cerebral cortical development.
|
| pubmed:affiliation |
Department of Anatomy, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|