Linked Life Data

16420188

Source:http://linkedlifedata.com/resource/pubmed/id/16420188

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2006-4-13
pubmed:abstractText
A magnetic nanoparticle composite of CuTAPc (copper tetra-aminophthalocyanine)-Fe(3)O(4) was prepared by in situ complexation and was characterized by FTIR (Fourier-transform IR) spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, FESEM (field emission scanning electron microscopy) micrograph and hysteresis loop. The results showed that CuTAPc formed the covering layer on the surface of the composite. Using glutaraldehyde, laccase was covalently attached to the surface of the composite. When white-rot-fungus (Pycnoporus sanguineus) laccase was immobilized on the CuTAPc-Fe(3)O(4) nanoparticle composite in PBS, the optimal reaction pH was 5.0 and the optimal temperature was 0 degrees C. When 2.0 mg/ml laccase solution was used, the immobilization yield was 20%. The activity, K(m), k(cat)/K(m) and V(max) values of the immobilized laccase were 1.43 units/mg, 2.38 x 10(-5) mol/l, 2.1 x 10(3) mol(-1) x s(-1) x l and 1.19 x 10(-6) mol x l(-1) /x min(-1) respectively. The system exhibited maximum enzyme activity at pH 3.0 and at 45 degrees C. After storage at 4 degrees C for 1 month, the residual activity of the immobilized laccase was 85% of its initial activity, while that of free laccase was only 30%. During 240 min incubation at 55 degrees C, the activity of immobilized laccase quickly increased to a maximum after 150 min and then decreased to 95% in the next 90 min, while the activity of the free enzyme decreased monotonically to 28%. After five consecutive operations, the immobilized laccase still retained 80% of its initial activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0885-4513
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-100
pubmed:dateRevised
2007-3-21
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Immobilization of Pycnoporus sanguineus laccase on copper tetra-aminophthalocyanine-Fe(3)O(4) nanoparticle composite.
pubmed:affiliation
Key Laboratory of Fiber Optic Sensing Technology and Information Processing (Ministry of Education), Wuhan University of Technology, Wuhan 430070, People's Republic of China. fosrcwut@public.wh.hb.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't