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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
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|
pubmed:dateCreated |
2006-1-18
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|
pubmed:abstractText |
A general method has been developed to immobilize antibodies onto an array surface by employing fusion proteins consisting of an elastin domain with tunable hydrophobic properties and an antibody-binding domain with high binding affinity and specificity for antibodies. Antibodies conjugated with the elastin fusion proteins can be directly printed on a self-assembled monolayer-modified glass slide in a functionally active orientation with a spatially defined pattern. An antibody array sensor for detection of tumor markers was fabricated to demonstrate the utility of the method. We expect that the method presented here could be a simple and universal platform to immobilize antibodies for the fabrication of a variety of antibody array sensors.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Jan
|
|
pubmed:issn |
0002-7863
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
25
|
|
pubmed:volume |
128
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
676-7
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|
pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
|
|
pubmed:year |
2006
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|
pubmed:articleTitle |
Fabrication of antibody arrays using thermally responsive elastin fusion proteins.
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|
pubmed:affiliation |
Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|
