Linked Life Data

16415079

Source:http://linkedlifedata.com/resource/pubmed/id/16415079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-5-11
pubmed:abstractText
We investigated changes in calcium concentration in cultured bovine aortic endothelial cells (BAECs) and rat adrenomedulary endothelial cells (RAMECs, microvascular) in response to different levels of shear stress. In BAECs, the onset of shear stress elicited a transient increase in intracellular calcium concentration that was spatially uniform, synchronous, and dose dependent. In contrast, the response of RAMECs was heterogeneous in time and space. Shear stress induced calcium waves that originated from one or several cells and propagated to neighboring cells. The number and size of the responding groups of cells did not depend on the magnitude of shear stress or the magnitude of the calcium change in the responding cells. The initiation and the propagation of calcium waves in RAMECs were significantly suppressed under conditions in which either purinergic receptors were blocked by suramin or extracellular ATP was degraded by apyrase. Exogenously applied ATP produced similarly heterogeneous responses. The number of responding cells was dependent on ATP concentration, but the magnitude of the calcium change was not. Our data suggest that shear stress stimulates RAMECs to release ATP, causing the increase in intracellular calcium concentration via purinergic receptors in cells that are heterogeneously sensitive to ATP. The propagation of the calcium signal is also mediated by ATP, and the spatial pattern suggests a locally elevated ATP concentration in the vicinity of the initially responding cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0363-6135
pubmed:author
pubmed:issnType
Print
pubmed:volume
290
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H2498-508
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16415079-Adenosine Triphosphate, pubmed-meshheading:16415079-Adrenal Medulla, pubmed-meshheading:16415079-Aniline Compounds, pubmed-meshheading:16415079-Animals, pubmed-meshheading:16415079-Aorta, Thoracic, pubmed-meshheading:16415079-Apyrase, pubmed-meshheading:16415079-Calcium, pubmed-meshheading:16415079-Calcium Signaling, pubmed-meshheading:16415079-Capillaries, pubmed-meshheading:16415079-Cell Communication, pubmed-meshheading:16415079-Endothelial Cells, pubmed-meshheading:16415079-Heptanol, pubmed-meshheading:16415079-Image Processing, Computer-Assisted, pubmed-meshheading:16415079-Immunohistochemistry, pubmed-meshheading:16415079-Physical Stimulation, pubmed-meshheading:16415079-Rats, pubmed-meshheading:16415079-Receptors, Purinergic P2, pubmed-meshheading:16415079-Receptors, Purinergic P2Y2, pubmed-meshheading:16415079-Stress, Mechanical, pubmed-meshheading:16415079-Suramin, pubmed-meshheading:16415079-Xanthenes
pubmed:year
2006
pubmed:articleTitle
Heterogeneous response of microvascular endothelial cells to shear stress.
pubmed:affiliation
School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural