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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2006-1-24
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pubmed:abstractText |
The aim of this study is to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres containing a staphylokinase variant K35R (DGR) with purpose of preserving the protein stability during both encapsulation and drug release. DGR-loaded microspheres are fabricated using a double-emulsion solvent extraction technique. Prior to encapsulation, the effect of ultrasonication emulsification of DGR solutions with methylene chloride on protein recovery was investigated. Moderate ultrasonic treatment of aqueous DGR/dichloromethane mixtures caused approximately 84% DGR aggregation. Polyvinyl alcohol (PVA) added into aqueous DGR solutions significantly improved DGR recovery to >90%. The effects of co-encapsulated PVA and NaCl in the external aqueous phase on the characteristics of the microspheres were investigated. When 2% PVA was co-encapsulated and 2.5% NaCl was added to the external water phase, DGR encapsulation efficiency was significantly increased from 7.1% to 78.1% and DGR was distributed uniformly throughout the microspheres. In vitro release test showed that DGR was released from PLGA microspheres in a sustained manner over 15 days. A large amount of released DGR was inactive in the absence of co-encapsulated PVA. On the contrary, when 2% PVA was co-encapsulated, the released DGR was almost completely intact within 9 days. In conclusion, PLGA microspheres can be an effective carrier for DGR and form a promising depot system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Delayed-Action Preparations,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Excipients,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Methylene Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Polyvinyl Alcohol,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/auR protein, Staphylococcus aureus,
http://linkedlifedata.com/resource/pubmed/chemical/polylactic acid-polyglycolic acid...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0378-5173
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
309
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16413979-Chemistry, Pharmaceutical,
pubmed-meshheading:16413979-Delayed-Action Preparations,
pubmed-meshheading:16413979-Drug Carriers,
pubmed-meshheading:16413979-Enzyme Stability,
pubmed-meshheading:16413979-Excipients,
pubmed-meshheading:16413979-Lactic Acid,
pubmed-meshheading:16413979-Metalloendopeptidases,
pubmed-meshheading:16413979-Methylene Chloride,
pubmed-meshheading:16413979-Microspheres,
pubmed-meshheading:16413979-Particle Size,
pubmed-meshheading:16413979-Polyglycolic Acid,
pubmed-meshheading:16413979-Polymers,
pubmed-meshheading:16413979-Polyvinyl Alcohol,
pubmed-meshheading:16413979-Protein Denaturation,
pubmed-meshheading:16413979-Solubility,
pubmed-meshheading:16413979-Solvents,
pubmed-meshheading:16413979-Surface Properties,
pubmed-meshheading:16413979-Technology, Pharmaceutical,
pubmed-meshheading:16413979-Time Factors,
pubmed-meshheading:16413979-Ultrasonics
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pubmed:year |
2006
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pubmed:articleTitle |
Stabilization and encapsulation of a staphylokinase variant (K35R) into poly(lactic-co-glycolic acid) microspheres.
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pubmed:affiliation |
Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, 130 Dong-an Road, Shanghai 200032, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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