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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-1-13
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pubmed:abstractText |
RNA loop-loop interactions are a prevalent motif in the formation of tertiary structure and are well suited to trigger molecular recognition between RNA molecules. We determined the stabilities of several loop-loop interactions with a constant 6 bp core sequence and varying unpaired flanking nucleotides and found that the flanking bases have a strong influence on the stability and ion dependence of the kissing complex. In general, the stabilities determined in 1 M Na+ are equivalent to those in the presence of near physiological Mg2+ concentrations. Therefore we further tested whether the stabilities determined in vitro and within yeast cells correlate, using a recently developed yeast RNA-hybrid system. For the majority of the loop types analyzed here, the melting temperatures determined in vitro are in good agreement with the relative beta-galactosidase activity in yeast cells, showing that data derived from in vitro measurements reflect in vivo properties. The most stable interactions are the naturally occurring HIV-1 DIS MAL and LAI derived loops with the motif (5' A(A)/(G)N6A 3'), emphasizing the crucial role of stable kissing complexes in HIV genome dimerization.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10329189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10411138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10550208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10606271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10623557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10937989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-10999604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-11493002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-11702070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-11931231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12054889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12127447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12356744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12458085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12853646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-12869711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-14734802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-15193319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-1524417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-15362866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-15459283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-15989962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-16301599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-3897553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-4519026,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-9241415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-9292496,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16410613-9778347
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
334-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16410613-Base Pairing,
pubmed-meshheading:16410613-HIV-1,
pubmed-meshheading:16410613-Nucleic Acid Conformation,
pubmed-meshheading:16410613-Nucleic Acid Denaturation,
pubmed-meshheading:16410613-Nucleotides,
pubmed-meshheading:16410613-Osmolar Concentration,
pubmed-meshheading:16410613-RNA, Viral,
pubmed-meshheading:16410613-RNA Stability,
pubmed-meshheading:16410613-Spectrophotometry, Ultraviolet,
pubmed-meshheading:16410613-Temperature,
pubmed-meshheading:16410613-beta-Galactosidase
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pubmed:year |
2006
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pubmed:articleTitle |
Stabilities of HIV-1 DIS type RNA loop-loop interactions in vitro and in vivo.
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pubmed:affiliation |
Max F. Perutz Laboratories, Department of Biochemistry, University of Vienna, Dr Bohrgasse 9/5, A-1030 Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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