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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-3-13
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pubmed:abstractText |
Herpes simplex viruses (HSVs) are able to hijack the host-cell IkappaB kinase (IKK)/NF-kappaB pathway, which regulates critical cell functions from apoptosis to inflammatory responses; however, the molecular mechanisms involved and the outcome of the signaling dysregulation on the host-virus interaction are mostly unknown. Here we show that in human keratinocytes HSV-1 attains a sophisticated control of the IKK/NF-kappaB pathway, inducing two distinct temporally controlled waves of IKK activity and disrupting the NF-kappaB autoregulatory mechanism. Using chromatin immunoprecipitation we demonstrate that dysregulation of the NF-kappaB-response is mediated by a virus-induced block of NF-kappaB recruitment to the promoter of the IkappaBalpha gene, encoding the main NF-kappaB-inhibitor. We also show that HSV-1 redirects NF-kappaB recruitment to the promoter of ICP0, an immediate-early viral gene with a key role in promoting virus replication. The results reveal a new level of control of cellular functions by invading viruses and suggest that persistent NF-kappaB activation in HSV-1-infected cells, rather than being a host response to the virus, may play a positive role in promoting efficient viral replication.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Vmw110 protein, Human herpesvirus 1,
http://linkedlifedata.com/resource/pubmed/chemical/prostaglandin A1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7110-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16407234-Blotting, Western,
pubmed-meshheading:16407234-Cell Line,
pubmed-meshheading:16407234-Chromatin Immunoprecipitation,
pubmed-meshheading:16407234-DNA Primers,
pubmed-meshheading:16407234-Gene Expression Regulation,
pubmed-meshheading:16407234-Genes, Viral,
pubmed-meshheading:16407234-Herpesvirus 1, Human,
pubmed-meshheading:16407234-Humans,
pubmed-meshheading:16407234-I-kappa B Proteins,
pubmed-meshheading:16407234-Immediate-Early Proteins,
pubmed-meshheading:16407234-Inflammation,
pubmed-meshheading:16407234-Keratinocytes,
pubmed-meshheading:16407234-Models, Genetic,
pubmed-meshheading:16407234-NF-kappa B,
pubmed-meshheading:16407234-Plasmids,
pubmed-meshheading:16407234-Promoter Regions, Genetic,
pubmed-meshheading:16407234-Prostaglandins A,
pubmed-meshheading:16407234-RNA, Messenger,
pubmed-meshheading:16407234-Signal Transduction,
pubmed-meshheading:16407234-Simplexvirus,
pubmed-meshheading:16407234-Time Factors,
pubmed-meshheading:16407234-Transcription, Genetic,
pubmed-meshheading:16407234-Transfection,
pubmed-meshheading:16407234-Ubiquitin-Protein Ligases,
pubmed-meshheading:16407234-Ultraviolet Rays
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pubmed:year |
2006
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pubmed:articleTitle |
Herpes simplex virus disrupts NF-kappaB regulation by blocking its recruitment on the IkappaBalpha promoter and directing the factor on viral genes.
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pubmed:affiliation |
Department of Biology and Dermatology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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