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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-2-3
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pubmed:abstractText |
Here, we report in vitro generation of Math1+ cerebellar granule cell precursors and Purkinje cells from ES cells by using soluble patterning signals. When neural progenitors induced from ES cells in a serum-free suspension culture are subsequently treated with BMP4 and Wnt3a, a significant proportion of these neural cells become Math1+. The induced Math1+ cells are mitotically active and express markers characteristic of granule cell precursors (Pax6, Zic1, and Zipro1). After purification by FACS and coculture with postnatal cerebellar neurons, ES cell-derived Math1+ cells exhibit typical features of neurons of the external granule cell layer, including extensive motility and a T-shaped morphology. Interestingly, differentiation of L7+/Calbindin-D28K+ neurons (characteristic of Purkinje cells) is induced under similar culture conditions but exhibits a higher degree of enhancement by Fgf8 rather than by Wnt3a. This is the first report of in vitro recapitulation of early differentiation of cerebellar neurons by using the ES cell system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bmp4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 4,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Fgf8 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 8,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3A Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3a protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
290
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
287-96
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16406324-Animals,
pubmed-meshheading:16406324-Bone Morphogenetic Protein 4,
pubmed-meshheading:16406324-Bone Morphogenetic Proteins,
pubmed-meshheading:16406324-Cell Culture Techniques,
pubmed-meshheading:16406324-Cell Differentiation,
pubmed-meshheading:16406324-Cell Separation,
pubmed-meshheading:16406324-Cell Transplantation,
pubmed-meshheading:16406324-Cells, Cultured,
pubmed-meshheading:16406324-Cerebellum,
pubmed-meshheading:16406324-Culture Media, Serum-Free,
pubmed-meshheading:16406324-Embryo, Mammalian,
pubmed-meshheading:16406324-Fibroblast Growth Factor 8,
pubmed-meshheading:16406324-Flow Cytometry,
pubmed-meshheading:16406324-Green Fluorescent Proteins,
pubmed-meshheading:16406324-Immunohistochemistry,
pubmed-meshheading:16406324-Mice,
pubmed-meshheading:16406324-Microscopy, Fluorescence,
pubmed-meshheading:16406324-Neurons,
pubmed-meshheading:16406324-Purkinje Cells,
pubmed-meshheading:16406324-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16406324-Stem Cells,
pubmed-meshheading:16406324-Wnt Proteins,
pubmed-meshheading:16406324-Wnt3 Protein,
pubmed-meshheading:16406324-Wnt3A Protein
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pubmed:year |
2006
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pubmed:articleTitle |
Generation of cerebellar neuron precursors from embryonic stem cells.
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pubmed:affiliation |
Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo, Kobe 650-0047, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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