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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-1-30
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pubmed:abstractText |
Upon injury, astrocytes assume an activated state associated with the release of inflammatory mediators. To model this, we stimulated murine primary astrocytes with a complete inflammatory cytokine mix consisting of TNF-alpha, IL-1beta and IFN-gamma. We analysed the transcriptional response of 480 genes at 4 and 16 h after stimulation on a chip designed to give a representative overview over the inflammation-relevant part of the transcriptome of macrophage-like cells. The list of the 182 genes found to be significantly regulated in astrocytes revealed an intriguing co-ordinate regulation of genes linked to the biological processes of antiviral/antimicrobial defence, antigen presentation and facilitation of leucocyte invasion. The latter group was characterized by very high up-regulations of chemokine genes. We also identified regulations of a thymidylate kinase and an interferon-regulated protein with a tetratricopeptide motive, both up to now only known from macrophages. The transcriptional regulations were confirmed on the protein level by a proteomic analysis. These findings taken together suggest that activated astrocytes in brain behave similarly in many respects to inflamed macrophages in the periphery.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
893-907
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16405499-Animals,
pubmed-meshheading:16405499-Animals, Newborn,
pubmed-meshheading:16405499-Astrocytes,
pubmed-meshheading:16405499-Cell Cycle,
pubmed-meshheading:16405499-Cell Death,
pubmed-meshheading:16405499-Cells, Cultured,
pubmed-meshheading:16405499-Cerebral Cortex,
pubmed-meshheading:16405499-Chemokine CCL5,
pubmed-meshheading:16405499-Cytokines,
pubmed-meshheading:16405499-Disease Models, Animal,
pubmed-meshheading:16405499-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16405499-Female,
pubmed-meshheading:16405499-Glial Fibrillary Acidic Protein,
pubmed-meshheading:16405499-Immunity, Innate,
pubmed-meshheading:16405499-Immunohistochemistry,
pubmed-meshheading:16405499-Inflammation,
pubmed-meshheading:16405499-Mice,
pubmed-meshheading:16405499-Mice, Inbred C57BL,
pubmed-meshheading:16405499-Models, Immunological,
pubmed-meshheading:16405499-Nitrites,
pubmed-meshheading:16405499-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16405499-Pregnancy,
pubmed-meshheading:16405499-Proteomics,
pubmed-meshheading:16405499-RNA, Messenger,
pubmed-meshheading:16405499-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16405499-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16405499-Stress, Physiological,
pubmed-meshheading:16405499-Time Factors
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pubmed:year |
2006
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pubmed:articleTitle |
The inflammatory transcriptome of reactive murine astrocytes and implications for their innate immune function.
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pubmed:affiliation |
Institute of Neuropathology, University of Zürich, Schmelzbergstrasse 12, CH-8032 Zürich, Switzerland. jeppefalsig.pedersen@usz.ch
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pubmed:publicationType |
Journal Article,
Comparative Study
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