Source:http://linkedlifedata.com/resource/pubmed/id/16403501
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2006-4-24
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| pubmed:abstractText |
Using RT-PCR, two cDNAs encoding preprotemporins were cloned from a total RNA preparation of the skin of Tago's brown frog Rana tagoi. Preprotemporin-1TGa cDNA directs the synthesis of temporin-1TGa (FLPILGKLLSGIL.NH2) previously isolated from R. tagoi skin. Preprotemporin-1TGb cDNA directs the synthesis of a novel 16-amino-acid-residue peptide (AVDLAKIANKVLSSLF.NH2) that, atypically for members of the temporin family, inhibits the growth of Gram-negative bacteria more effectively than Gram-positive bacteria. Preprotemporin-1TGa mRNA and preprotemporin-1TGb mRNA were not detected in skin prior to the onset of metamorphosis (stage 35) but the levels of the transcripts increased markedly during metamorphosis reaching a maximum at stage 38. Exposure of adult animals to 10(-8) M triiodothyronine (T3) for 72 h enhanced expression of the preprotemporin-1TGb gene (approximately threefold) but did not significantly change the level of expression of the preprotemporin-1TGa gene. Exposure of the animals to 10(-8) M T3 and 10(-6) M bisphenol A, an endocrine disrupting chemical that potently inhibits the action of thyroid hormones (THs), reduced expression of the preprotemporin-1TGb gene by 10-fold and the preprotemporin-1TGa gene by threefold. We propose that T3-stimulated synthesis of antimicrobial peptides is important in protecting the animal against microorganisms, particularly at metamorphosis and during skin moulting, but environmental pollutants can inhibit peptide synthesis and render the animal susceptible to invasion by pathogens.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Infective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Triiodothyronine,
http://linkedlifedata.com/resource/pubmed/chemical/bisphenol A,
http://linkedlifedata.com/resource/pubmed/chemical/temporin
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0016-6480
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
146
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
242-50
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16403501-Amino Acid Sequence,
pubmed-meshheading:16403501-Animals,
pubmed-meshheading:16403501-Anti-Infective Agents,
pubmed-meshheading:16403501-Base Sequence,
pubmed-meshheading:16403501-Cloning, Molecular,
pubmed-meshheading:16403501-DNA, Complementary,
pubmed-meshheading:16403501-Erythrocytes,
pubmed-meshheading:16403501-Gene Expression Regulation, Developmental,
pubmed-meshheading:16403501-Hemolysis,
pubmed-meshheading:16403501-Humans,
pubmed-meshheading:16403501-Lethal Dose 50,
pubmed-meshheading:16403501-Metamorphosis, Biological,
pubmed-meshheading:16403501-Microbial Sensitivity Tests,
pubmed-meshheading:16403501-Molecular Sequence Data,
pubmed-meshheading:16403501-Phenols,
pubmed-meshheading:16403501-Protein Precursors,
pubmed-meshheading:16403501-Proteins,
pubmed-meshheading:16403501-Ranidae,
pubmed-meshheading:16403501-Triiodothyronine
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| pubmed:year |
2006
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| pubmed:articleTitle |
Developmental and triiodothyronine-induced expression of genes encoding preprotemporins in the skin of Tago's brown frog Rana tagoi.
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| pubmed:affiliation |
Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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