Source:http://linkedlifedata.com/resource/pubmed/id/16398500
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2006-1-9
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| pubmed:abstractText |
Structures of three pectic arabinogalactans, one from Vernonia kotschyana (Vk2a) and two from Cochlospermum tinctorium (Ct50A1 and Ct50A2), and their complement fixation and induction of B cell proliferation in vitro were compared. The polysaccharide Vk2a expressed potent biological activity in both assays compared with Ct50A1 and Ct50A2. Vk2a possessed a very high molecular weight (1150 +/- 20 kDa) compared with Ct50A1 and Ct50A2 which both showed a polydisperse nature with the highest molecular weight polymers in each fraction estimated at approximately 105 kDa (Ct1a) and 640 +/- 100 kDa (Ct2a), respectively. The HMW polymers showed complement fixation in the same range as the native fractions. The arabinogalactan II content was low in Vk2a (2%) compared with that in Ct50A1 (23%) and Ct50A2 (12%). The high molecular weight polymers were subjected to digestion with a beta-d-(1, 3)-galactanase-rich fraction from Driselase, oligomers were isolated by HPAEC, and their finer structures were determined by MALDI- and ES-qoToF-MS, linkage, and monosaccharide composition analyses. Vk2a consists of both a galacturonan core and a rhamnogalacturonan core rich in neutral side chains. The backbones of both Ct-polysaccharides consist mainly of RG-I regions with numerous neutral side chains dominated by galactosyl residues, whereas the homogalacturonan regions seem to be small. Differences in the chain lengths of the 6-linked galacto-oligosaccharides attached to the 3-linked galactan core could not be related to the differences in the potencies of the biological activities observed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Ulcer Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Galactans,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Pectins,
http://linkedlifedata.com/resource/pubmed/chemical/arabinogalactan
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1525-7797
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| pubmed:author |
pubmed-author:DialloDrissaD,
pubmed-author:KiyoharaHiroakiH,
pubmed-author:MatsumotoTsukasaT,
pubmed-author:MichaelsenTerje ETE,
pubmed-author:NergardCecilie SognCS,
pubmed-author:PatelTrusharT,
pubmed-author:PaulsenBerit SmestadBS,
pubmed-author:PetersenDirkD,
pubmed-author:ReynoldsJames CJC,
pubmed-author:Thomas-OatesJane EJE,
pubmed-author:YamadaHarukiH
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| pubmed:issnType |
Print
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| pubmed:volume |
7
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
71-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16398500-Amino Acids,
pubmed-meshheading:16398500-Animals,
pubmed-meshheading:16398500-Anti-Ulcer Agents,
pubmed-meshheading:16398500-B-Lymphocytes,
pubmed-meshheading:16398500-Bixaceae,
pubmed-meshheading:16398500-Cells, Cultured,
pubmed-meshheading:16398500-Complement Fixation Tests,
pubmed-meshheading:16398500-Complement System Proteins,
pubmed-meshheading:16398500-Female,
pubmed-meshheading:16398500-Galactans,
pubmed-meshheading:16398500-Glycosylation,
pubmed-meshheading:16398500-Magnetic Resonance Spectroscopy,
pubmed-meshheading:16398500-Mice,
pubmed-meshheading:16398500-Mitogens,
pubmed-meshheading:16398500-Mitosis,
pubmed-meshheading:16398500-Molecular Weight,
pubmed-meshheading:16398500-Monosaccharides,
pubmed-meshheading:16398500-Oceans and Seas,
pubmed-meshheading:16398500-Pectins,
pubmed-meshheading:16398500-Structure-Activity Relationship,
pubmed-meshheading:16398500-Vernonia
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| pubmed:year |
2006
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| pubmed:articleTitle |
Structures and structure-activity relationships of three mitogenic and complement fixing pectic arabinogalactans from the malian antiulcer plants Cochlospermum tinctorium A. Rich and Vernonia kotschyana Sch. Bip. ex Walp.
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| pubmed:affiliation |
Department of Pharmacognosy, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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