Source:http://linkedlifedata.com/resource/pubmed/id/16397243
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2006-1-6
|
| pubmed:abstractText |
We used dual-color in vivo cellular imaging to visualize trafficking, nuclear-cytoplasmic dynamics, and the viability of cancer cells after their injection into the portal vein of mice. For these studies, we used dual-color fluorescent cancer cells that express green fluorescent protein (GFP) linked to histone H2B in the nucleus and retroviral red fluorescent protein (RFP) in the cytoplasm. Human HCT-116-GFP-RFP colon cancer and mouse mammary tumor (MMT) cells were HCT-116-GFP-RFP in the portal vein of nude mice. The cells were observed intravitally in the liver at the single-cell level using the Olympus OV100 whole-mouse imaging system. Most HCT-116-GFP-RFP cells remained in sinusoids near peripheral portal veins. Only a small fraction of the cancer cells invaded the lobular area. Extensive clasmocytosis (destruction of the cytoplasm) of the HCT-116-GFP-RFP cells occurred within 6 hours. The number of apoptotic cells rapidly increased within the portal vein within 12 hours of injection. Apoptosis was readily visualized in the dual-color cells by their altered nuclear morphology. The data suggest rapid death of HCT-116-GFP-RFP cells in the portal vein. In contrast, dual-color MMT-GFP-RFP cells injected into the portal vein mostly survived in the liver of nude mice 24 hours after injection. Many surviving MMT-GFP-RFP cells showed invasive figures with cytoplasmic protrusions. The cells grew aggressively and formed colonies in the liver. However, when the host mice were pretreated with cyclophosphamide, the HCT-116-GFP-RFP cells also survived and formed colonies in the liver after portal vein injection. These results suggest that a cyclophosphamide-sensitive host cellular system attacked the HCT-116-GFP-RFP cells but could not effectively kill the MMT-GFP-RFP cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
66
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
303-6
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:16397243-Animals,
pubmed-meshheading:16397243-Cell Growth Processes,
pubmed-meshheading:16397243-Cell Nucleus,
pubmed-meshheading:16397243-Cell Survival,
pubmed-meshheading:16397243-Colonic Neoplasms,
pubmed-meshheading:16397243-Cyclophosphamide,
pubmed-meshheading:16397243-Cytoplasm,
pubmed-meshheading:16397243-Green Fluorescent Proteins,
pubmed-meshheading:16397243-HCT116 Cells,
pubmed-meshheading:16397243-Humans,
pubmed-meshheading:16397243-Image Processing, Computer-Assisted,
pubmed-meshheading:16397243-Liver Neoplasms, Experimental,
pubmed-meshheading:16397243-Luminescent Proteins,
pubmed-meshheading:16397243-Male,
pubmed-meshheading:16397243-Mammary Neoplasms, Experimental,
pubmed-meshheading:16397243-Mice,
pubmed-meshheading:16397243-Mice, Nude,
pubmed-meshheading:16397243-NIH 3T3 Cells,
pubmed-meshheading:16397243-Neoplastic Cells, Circulating,
pubmed-meshheading:16397243-Portal Vein
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area.
|
| pubmed:affiliation |
AntiCancer, Inc., San Diego, California 92111, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|