Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-8-28
pubmed:abstractText
Forty-five consecutive patients with various gastrointestinal disorders were identified as having Shiga-like toxin (SLT)-producing Escherichia coli infections. This was shown by the cytotoxic effect of stool extracts in Vero cell cultures which was neutralizable by antibodies to SLTs and by isolation of E. coli that hybridized with DNA probes complementary to SLT-I and SLT-II sequences. When we tested the same strains for SLT genes after subcultivation, the isolates from 15 patients became negative by colony hybridization and polymerase chain reaction and failed to produce SLTs. The instability of SLT genes warrants direct screening methods for clinical material and the development of new culture methods to prevent the loss of SLT genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-1500528, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-1559006, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-1757536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-1997410, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2464885, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2644022, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2656395, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2666436, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2687320, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-2842369, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3045088, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3063914, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-338490, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3522426, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3546519, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3886804, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-3905611, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-6135046, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-6144957, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-6387911, http://linkedlifedata.com/resource/pubmed/commentcorrection/1639518-6418852
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3464-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation.
pubmed:affiliation
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't