Linked Life Data

1639217

Source:http://linkedlifedata.com/resource/pubmed/id/1639217

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1992-8-28
pubmed:abstractText
The nerve growth factor (NGF) content of the mouse submandibular gland (SMG) is under hormonal control and is modulated by both thyroid hormones (TH) and androgens. The sexual dimorphism of the gland is well documented. In the adult male mouse, the SMG contains 10 times more NGF compared to the female. Conversely, castration of male mice reduces the SMG NGF levels to those found in control females. In order to determine the locus at which androgens and TH exert their effect on NGF gene expression in the SMG, steady-state NGF mRNA levels were determined. Daily treatment of adult female mice with TH for 1 week increased NGF mRNA levels 6-fold. Androgen treatment produced a 20-fold increase in SMG NGF mRNA, which was comparable to levels detected in the control adult male SMG. The effect of TH on NGF mRNA levels was time-dependent and coincided with the increase in NGF protein concentrations. At 48 h after a single TH injection, NGF mRNA levels (measured in SMG total RNA) increased 2-4-fold, while heteronuclear (hn) RNA levels were increased 1.5-2-fold. The NGF gene transcription rate was determined by run-on assay following TH treatment. A small but significant 2-fold induction by TH of NGF gene transcription was found at 24-48 h. Cytoplasmic RNA prepared from the same SMGs used in the run-on experiments was tested by S1 nuclease protection; NGF cytoplasmic RNA was increased 7-fold in the SMGs of females treated with TH 48 h previously. These results demonstrate that the effect of TH on NGF gene expression is due in part to an induction of NGF gene transcription. The discrepancies observed between transcription rate and mRNA levels suggest that the major effect of TH is at the post-transcriptional level, possibly mRNA stabilization. The time required to observe an induction of TH on NGF gene transcription is suggestive of an indirect effect, possibly through the induction by TH of another protein which in turn activates the NGF gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-54
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:1639217-Anabolic Agents, pubmed-meshheading:1639217-Analysis of Variance, pubmed-meshheading:1639217-Animals, pubmed-meshheading:1639217-Blotting, Northern, pubmed-meshheading:1639217-Female, pubmed-meshheading:1639217-Gene Expression, pubmed-meshheading:1639217-Mice, pubmed-meshheading:1639217-Nerve Growth Factors, pubmed-meshheading:1639217-Nucleic Acid Hybridization, pubmed-meshheading:1639217-RNA, Messenger, pubmed-meshheading:1639217-RNA Processing, Post-Transcriptional, pubmed-meshheading:1639217-Radioimmunoassay, pubmed-meshheading:1639217-Submandibular Gland, pubmed-meshheading:1639217-Testosterone, pubmed-meshheading:1639217-Thyroxine, pubmed-meshheading:1639217-Time Factors, pubmed-meshheading:1639217-Transcription, Genetic, pubmed-meshheading:1639217-Triiodothyronine, pubmed-meshheading:1639217-Tubulin
pubmed:year
1992
pubmed:articleTitle
Thyroid hormone and androgen regulation of nerve growth factor gene expression in the mouse submandibular gland.
pubmed:affiliation
Endocrine Research Laboratories, Loeb Institute for Medical Research, Ottawa Civic Hospital, Canada.
pubmed:publicationType
Journal Article