Linked Life Data

16391851

Source:http://linkedlifedata.com/resource/pubmed/id/16391851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-1-4
pubmed:abstractText
Multicellular tumor spheroid (MTS) represents a three-dimensional structural form of tumors in laboratory conditions, and it has the characteristics of avascular micrometastases or intervascular spaces of big tumors. Recent studies indicate that extracellular matrix (ECM) proteins play a critical role in tumor metastasis, therefore normal and cancer cells require an ECM for survival, proliferation and differentiation. Doxorubicin and Docetaxel are widely used in the therapy of breast cancer, as well as in in vivo and in vitro studies. In this study, we examined the effect of apoptosis and proliferation of cells on the human breast cancer cell line, MCF-7, by using p53, bcl-2 and Ki67 gene expression, and the tendency to metastasis with extracellular matrix proteins, laminin and type IV collagen after chemotherapy in the spheroid model. The apoptotic cell death in situ was detected by TUNEL method. TUNEL-positive cells and positive immunoreactivities of laminin, type IV collagen, p53 and, bcl-2 were detected in the control group. There was no laminin and type IV collagen immunoreactivities in spheroids of drug groups. While TUNEL-positive cells and p53 immunoreactivity were detected in Docetaxel, Doxorubicin and Docetaxel/Doxorubicin groups, p53 immunoreactivity was not observed in the Docetaxel group. There was no bcl-2 immunoreactivity in either drug group. In addition, we did not detect Ki67 immunoreactivity in both control and drug treatment groups. However, the absence of Ki67 protein in MCF-7 breast multicellular tumor spheroids is possibly related to the cells in G0 or S phase. These chemotherapeutic agents may affect the presence of ECM proteins in this in vitro model of micrometastasis of spheroids. These findings suggest that the possible mechanism of cell death in Doxorubicin and Docetaxel/Doxorubicin treatment groups is related to apoptosis through the p53 pathway. However, we considered the possibility that there is another control mechanism for the Docetaxel group.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-40
pubmed:meshHeading
pubmed-meshheading:16391851-Antineoplastic Agents, pubmed-meshheading:16391851-Apoptosis, pubmed-meshheading:16391851-Collagen Type IV, pubmed-meshheading:16391851-Doxorubicin, pubmed-meshheading:16391851-Drug Therapy, Combination, pubmed-meshheading:16391851-Extracellular Matrix Proteins, pubmed-meshheading:16391851-Female, pubmed-meshheading:16391851-Gene Expression, pubmed-meshheading:16391851-Genes, bcl-2, pubmed-meshheading:16391851-Genes, p53, pubmed-meshheading:16391851-Humans, pubmed-meshheading:16391851-Immunohistochemistry, pubmed-meshheading:16391851-In Situ Nick-End Labeling, pubmed-meshheading:16391851-Ki-67 Antigen, pubmed-meshheading:16391851-Laminin, pubmed-meshheading:16391851-Spheroids, Cellular, pubmed-meshheading:16391851-Taxoids, pubmed-meshheading:16391851-Tumor Cells, Cultured
pubmed:year
2006
pubmed:articleTitle
Effect of apoptosis and response of extracellular matrix proteins after chemotherapy application on human breast cancer cell spheroids.
pubmed:affiliation
Department of Histology and Embryology, Ege University School of Medicine, TR-35100 izmir, Turkey. goktem@med.ege.edu.tr
pubmed:publicationType
Journal Article