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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1975-6-13
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pubmed:abstractText |
HEp-2 cells infected with herpes simplex 1 virus contain RNA transcripts capable of forming double-stranded (DS) RNA on annealing. The properties of purified DS RNA were as follows (i) DS RNA is resistant to depolymerization by RNase A or T-1 in 2 times 0.15 M NaCl, plus 0.015 M sodium citrate (SSC) but not 0.1 times SSC or following thermal denaturation. (ii) The Tm of the viral DS RNA was 100 C in 0.1 times SSC. (iii) Undenatured DS RNA does not hybridize with viral DNA: upon denaturation, excess unlabeled RNA drove 50 to 55% of labeled DNA into DNA-RNA hybrid. The kinetics of hybridization indicate that the DS RNA consists of at least two populations of transcripts arising from 29 and 26% of viral DNA and differing 40-fold in molar concentration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-163344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4269245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4316015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4329966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4337161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4341693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4341703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4345508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4352971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4353523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-4373710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-5077859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-5335753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/163916-5789322
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
707-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:163916-Base Sequence,
pubmed-meshheading:163916-Carcinoma, Squamous Cell,
pubmed-meshheading:163916-Cell Line,
pubmed-meshheading:163916-Hot Temperature,
pubmed-meshheading:163916-Laryngeal Neoplasms,
pubmed-meshheading:163916-Nucleic Acid Denaturation,
pubmed-meshheading:163916-Nucleic Acid Hybridization,
pubmed-meshheading:163916-RNA, Viral,
pubmed-meshheading:163916-Simplexvirus,
pubmed-meshheading:163916-Transcription, Genetic,
pubmed-meshheading:163916-Virus Replication
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pubmed:year |
1975
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pubmed:articleTitle |
RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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