Source:http://linkedlifedata.com/resource/pubmed/id/16391521
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2006-1-4
|
| pubmed:abstractText |
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Here, we report the identification of an alternatively-spliced variant of nanog. This variant lacked a stretch of amino acids (residues 168-183) located between the HD and tryptophan-repeat (WR) of the previously-reported full length sequence, suggesting that the deleted sequence functions as a linker and possibly affects the flexibility of the C-terminal transactivation domain relative to the DNA binding domain. Expression of mRNA encoding the splice variant, designated as nanog-delta 48, was much lower than that of the full length version in human ES cells. The ratio of nanog-delta 48 transcript to full length transcript increased, however, in multipotent adult progenitor cells. EMSA analysis revealed that both forms of Nanog were able to bind a nanog binding sequence with roughly the same affinity. A reporter plasmid assay also showed that both variants of nanog modestly repressed transactivation of gata-4, whose expression is proposed to be inhibited by nanog, with comparable potency. We conclude that, despite the difference in primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NANOG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1226-3613
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
601-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:16391521-Alternative Splicing,
pubmed-meshheading:16391521-Amino Acid Sequence,
pubmed-meshheading:16391521-Base Sequence,
pubmed-meshheading:16391521-Cell Nucleus,
pubmed-meshheading:16391521-Cells, Cultured,
pubmed-meshheading:16391521-DNA-Binding Proteins,
pubmed-meshheading:16391521-Exons,
pubmed-meshheading:16391521-GATA4 Transcription Factor,
pubmed-meshheading:16391521-Gene Expression Profiling,
pubmed-meshheading:16391521-Genes, Reporter,
pubmed-meshheading:16391521-Homeodomain Proteins,
pubmed-meshheading:16391521-Humans,
pubmed-meshheading:16391521-Introns,
pubmed-meshheading:16391521-Molecular Sequence Data,
pubmed-meshheading:16391521-Promoter Regions, Genetic,
pubmed-meshheading:16391521-RNA, Messenger,
pubmed-meshheading:16391521-Transcriptional Activation,
pubmed-meshheading:16391521-Transfection
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog.
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| pubmed:affiliation |
Division of Molecular and Life Sciences, College of Science and Technology, Hanyang University, Seoul 133-791, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|