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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-4-11
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pubmed:abstractText |
Helper-dependent (HD) adenovirus (Ad) vectors, deleted of all viral coding sequences, have a higher cloning capacity, improved performance of tissue-specific promoters, and reduced toxicity in animals relative to first-generation Ad vectors, making these vectors promising tools for gene transfer in vitro and in vivo. However, the large size of HDAd precursor plasmids renders them relatively difficult to manipulate due to the paucity of unique restriction enzyme sites suitable for transgene insertion and to the size constraints imposed by the viral packaging machinery.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1099-498X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2006 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
442-51
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16389604-Adenoviridae,
pubmed-meshheading:16389604-Animals,
pubmed-meshheading:16389604-Base Sequence,
pubmed-meshheading:16389604-Breast Neoplasms,
pubmed-meshheading:16389604-Cell Line,
pubmed-meshheading:16389604-Cell Line, Tumor,
pubmed-meshheading:16389604-Gene Duplication,
pubmed-meshheading:16389604-Genetic Vectors,
pubmed-meshheading:16389604-Helper Viruses,
pubmed-meshheading:16389604-Humans,
pubmed-meshheading:16389604-Mammaglobin A,
pubmed-meshheading:16389604-Mice,
pubmed-meshheading:16389604-Molecular Sequence Data,
pubmed-meshheading:16389604-Neoplasm Proteins,
pubmed-meshheading:16389604-Organ Specificity,
pubmed-meshheading:16389604-Plasmids,
pubmed-meshheading:16389604-Promoter Regions, Genetic,
pubmed-meshheading:16389604-Sensitivity and Specificity,
pubmed-meshheading:16389604-Transduction, Genetic,
pubmed-meshheading:16389604-Uteroglobin
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pubmed:year |
2006
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pubmed:articleTitle |
A convenient plasmid system for construction of helper-dependent adenoviral vectors and its application for analysis of the breast-cancer-specific mammaglobin promoter.
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pubmed:affiliation |
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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