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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
15
|
| pubmed:dateCreated |
1992-8-28
|
| pubmed:abstractText |
Transfection of a neuroblastoma cell line with expression vectors containing two different segments of human c-myb complementary DNA in antisense orientation yielded far fewer transfectant clones than did the transfection with the identical segments in sense orientation. In cell clones expressing c-myb antisense RNA, levels of the c-myb protein were down-regulated and the proliferation rate was slower than that of cells transfected with sense constructs or the untransfected parental cell line. Treatment of neuroblastoma and neuroepithelioma cell lines with a c-myb antisense oligodeoxynucleotide strongly inhibited cell growth. These data indicate a definite involvement of c-myb in the proliferation of neuroectodermal tumor cells extending the role of this protooncogene beyond the hematopoietic system. The availability of cell clones that transcribe c-myb antisense RNA provides a useful tool to study the involvement of other genes in the proliferation and differentiation of neuroblastoma cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
52
|
| pubmed:geneSymbol |
c-myb
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4221-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1638535-Animals,
pubmed-meshheading:1638535-Base Sequence,
pubmed-meshheading:1638535-Cell Division,
pubmed-meshheading:1638535-Cell Line,
pubmed-meshheading:1638535-Cell Line, Transformed,
pubmed-meshheading:1638535-Cloning, Molecular,
pubmed-meshheading:1638535-Genetic Vectors,
pubmed-meshheading:1638535-Humans,
pubmed-meshheading:1638535-Molecular Sequence Data,
pubmed-meshheading:1638535-Neuroblastoma,
pubmed-meshheading:1638535-Oligodeoxyribonucleotides,
pubmed-meshheading:1638535-Oligonucleotides, Antisense,
pubmed-meshheading:1638535-Oncogenes,
pubmed-meshheading:1638535-Polymerase Chain Reaction,
pubmed-meshheading:1638535-Proto-Oncogenes,
pubmed-meshheading:1638535-RNA, Antisense,
pubmed-meshheading:1638535-RNA, Neoplasm,
pubmed-meshheading:1638535-Transcription, Genetic,
pubmed-meshheading:1638535-Transfection
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Inhibition of proliferation by c-myb antisense RNA and oligodeoxynucleotides in transformed neuroectodermal cell lines.
|
| pubmed:affiliation |
ENEA (Ente Nuove tecnologie Energia e Ambiente) CRE Casaccia, Division of Molecular Biology, Rome, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|