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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-1-25
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pubmed:abstractText |
AMP-activated kinase (AMPK) is a highly conserved heterotrimeric kinase that functions as a metabolic regulator of cellular enzymes involved in carbohydrate and fat metabolism, which regulate ATP conservation and synthesis. Here, we investigated whether AMPK signaling has a role in the regulation of angiotensin II (Ang II)-induced proliferation in rat cardiac fibroblasts. Aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR) activated AMPK in rat cardiac fibroblasts and increased Ang II-induced extracellular signal-regulated kinase 1/2 phosphorylation and activity. AICAR also increased Ang II-induced c-fos mRNA expression in the cells. [3H]-thymidine and [3H]-proline incorporation by cardiac fibroblasts treated with Ang II was enhanced when the cells were pretreated with AICAR. Inhibition of AMPK by small interfering RNA for AMPKalpha1 suppressed Ang II-induced extracellular signal-regulated kinase activity, c-fos mRNA expression, and cell proliferation. Treatment of rats with AICAR (1 mg/g body weight per day) for 1 week significantly enhanced Ang II-induced hypertrophy of the myocardium. Our findings indicate that AMPK works as a stimulator of the Ang II-induced proliferative pathway in cardiac fibroblasts. Inhibition of AMPK signaling might serve as a new therapeutic target of remodeling of the hypertrophic myocardium.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AICA ribonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/AMP-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoimidazole Carboxamide,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Proline,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1524-4563
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16380538-AMP-Activated Protein Kinases,
pubmed-meshheading:16380538-Aminoimidazole Carboxamide,
pubmed-meshheading:16380538-Angiotensin II,
pubmed-meshheading:16380538-Animals,
pubmed-meshheading:16380538-Cardiomegaly,
pubmed-meshheading:16380538-Cell Proliferation,
pubmed-meshheading:16380538-Cells, Cultured,
pubmed-meshheading:16380538-Drug Synergism,
pubmed-meshheading:16380538-Enzyme Activation,
pubmed-meshheading:16380538-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:16380538-Fibroblasts,
pubmed-meshheading:16380538-Multienzyme Complexes,
pubmed-meshheading:16380538-Myocardium,
pubmed-meshheading:16380538-Organ Size,
pubmed-meshheading:16380538-Proline,
pubmed-meshheading:16380538-Protein-Serine-Threonine Kinases,
pubmed-meshheading:16380538-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:16380538-RNA, Messenger,
pubmed-meshheading:16380538-RNA, Small Interfering,
pubmed-meshheading:16380538-Rats,
pubmed-meshheading:16380538-Rats, Wistar,
pubmed-meshheading:16380538-Ribonucleotides,
pubmed-meshheading:16380538-Signal Transduction,
pubmed-meshheading:16380538-Thymidine
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pubmed:year |
2006
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pubmed:articleTitle |
Activation of AMP-activated protein kinase enhances angiotensin ii-induced proliferation in cardiac fibroblasts.
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pubmed:affiliation |
Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japan. yhattori@dokkyomed.ac.jp
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pubmed:publicationType |
Journal Article
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