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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-9-3
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pubmed:abstractText |
A simple procedure for obtaining highly purified preparations of native monoclonal (Waldenström's disease) immunoglobulin M possessing a rheumatoid activity (IgM-RF) has been developed. The method is based on the use of affinity chromatography with a new readily available adsorbent (immunoglobulin G-porous glass) and 3 M LiCl in Tris-buffer pH 8.3-8.4 able to induce the dissociation of the IgM-RF-IgG complex. The IgM-RF preparation thus obtained was characterized in terms of amino acid composition (relative to conventional monoclonal IgM), carbohydrate composition and structure of oligosaccharide moieties of a complex type. It was shown that some dissociation conditions for the IgM-RF-IgG complex routinely used to isolate IgM-RF provoke irreversible denaturation of IgM-RF when applied to a preliminarily purified complex.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0320-9725
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
617-26
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:1637922-Amino Acids,
pubmed-meshheading:1637922-Antibodies, Monoclonal,
pubmed-meshheading:1637922-Carbohydrate Sequence,
pubmed-meshheading:1637922-Carbohydrates,
pubmed-meshheading:1637922-Chromatography, Affinity,
pubmed-meshheading:1637922-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1637922-Humans,
pubmed-meshheading:1637922-Immunodiffusion,
pubmed-meshheading:1637922-Immunoglobulin M,
pubmed-meshheading:1637922-Molecular Sequence Data,
pubmed-meshheading:1637922-Protein Denaturation,
pubmed-meshheading:1637922-Rheumatoid Factor
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pubmed:year |
1992
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pubmed:articleTitle |
[A simple method of isolating monoclonal immunoglobulin M, possessing rheumatoid activity].
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pubmed:publicationType |
Journal Article,
English Abstract
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