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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-2-6
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pubmed:abstractText |
IL-2-induced vascular leak syndrome (VLS) is an important mechanism explaining the toxic effects of this cytokine and limiting its therapeutic use. We previously characterized a mouse model of IL-2-induced pulmonary VLS used to demonstrate that NK lymphocytes are involved in early/acute phase VLS (after one IL-2 injection). We also showed that NK cells and polymorphonuclear neutrophils (PMN) are involved in the late/chronic phase of the syndrome (after four daily IL-2 injections). In this study we use our mouse model to evaluate the role played by the IL-2 receptor (IL-2R) in VLS induction. Mouse and human IL-2R are different since the mouse IL-2Rbeta chain does not recognize IL-2. Here, we compare the acute and late VLS responses in human IL-2Rbeta transgenic and C57BL/6 wild type mice. Parameters linked to early phase VLS (bronchoconstriction and PMN mobilization) are enhanced in human IL-2Rbeta transgenic mice. By contrast, parameters used to measure late events (protein leakage and edema) are similar in human IL-2Rbeta transgenic mice and C57BL/6 wild type animals. However, after four IL-2 injections, the cellular content of the bronchoalveolar lavage fluids was different between the two types of animals. This study also characterizes a humanized animal model that could be further used to study human IL-2 activity and side effects in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/IL2RB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il2rb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2 Receptor beta Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Methacholine Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
280-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16378732-Airway Resistance,
pubmed-meshheading:16378732-Animals,
pubmed-meshheading:16378732-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:16378732-Capillary Leak Syndrome,
pubmed-meshheading:16378732-Cell Count,
pubmed-meshheading:16378732-Cell Movement,
pubmed-meshheading:16378732-Gene Expression,
pubmed-meshheading:16378732-Humans,
pubmed-meshheading:16378732-Interleukin-2,
pubmed-meshheading:16378732-Interleukin-2 Receptor beta Subunit,
pubmed-meshheading:16378732-Lung,
pubmed-meshheading:16378732-Lymphocytes,
pubmed-meshheading:16378732-Male,
pubmed-meshheading:16378732-Methacholine Chloride,
pubmed-meshheading:16378732-Mice,
pubmed-meshheading:16378732-Mice, Inbred C57BL,
pubmed-meshheading:16378732-Mice, Transgenic,
pubmed-meshheading:16378732-Neutrophils,
pubmed-meshheading:16378732-Organ Size,
pubmed-meshheading:16378732-Peroxidase,
pubmed-meshheading:16378732-Plethysmography, Whole Body,
pubmed-meshheading:16378732-Proteins,
pubmed-meshheading:16378732-Receptors, Interleukin-2
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pubmed:year |
2005
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pubmed:articleTitle |
Constitutive expression of IL-2Rbeta chain and its effects on IL-2-induced vascular leak syndrome.
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pubmed:affiliation |
Unité de Pharmacologie Cellulaire, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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