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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0017837,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0065576,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0206131,
umls-concept:C1415341,
umls-concept:C1511938,
umls-concept:C1719914,
umls-concept:C2828406,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
2006-1-13
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pubmed:abstractText |
Western blot analysis of 3T3-L1 adipocyte proteins using an anti-C/EBPalpha antibody detected a 24kD polypeptide in addition to the expected 42 and 30kD isoforms of C/EBPalpha. Mass spectrometric sequencing of the protein following its purification by HPLC and preparative 2D gel electrophoresis identified it as glutathione S-transferase zeta/maleylacetoacetate isomerase (GSTzeta/MAAI). Expression of GSTzeta/MAAI mRNA and protein was induced during the terminal phase of adipogenesis in 3T3-L1 preadipocytes. Ectopic expression of PPARgamma2 in NIH-3T3 fibroblasts exposed to insulin and troglitazone-induced perilipin production, but was incapable of activating GSTzeta/MAAI unless C/EBPalpha was also expressed. Similarly, ectopic expression of C/EBPalpha in PPARgamma +/- or PPARgamma -/- MEFs demonstrated that the C/EBPalpha-dependent induction of GSTzeta/MAAI production was dependent on expression of endogenous PPARgamma. These data suggest a role for GSTzeta/MAAI in mature adipocytes that may be responsive to the thiazolidinedione class of insulin sensitizing PPARgamma ligands.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Gstz1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxisome Proliferator-Activated...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/cis-trans-Isomerases,
http://linkedlifedata.com/resource/pubmed/chemical/maleylacetoacetate isomerase
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
340
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
845-51
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16376852-3T3-L1 Cells,
pubmed-meshheading:16376852-Adipocytes,
pubmed-meshheading:16376852-Animals,
pubmed-meshheading:16376852-Antioxidants,
pubmed-meshheading:16376852-Blotting, Western,
pubmed-meshheading:16376852-CCAAT-Enhancer-Binding Protein-alpha,
pubmed-meshheading:16376852-Cell Differentiation,
pubmed-meshheading:16376852-Cells, Cultured,
pubmed-meshheading:16376852-Chromatography, High Pressure Liquid,
pubmed-meshheading:16376852-DNA, Complementary,
pubmed-meshheading:16376852-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:16376852-Fibroblasts,
pubmed-meshheading:16376852-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:16376852-Genetic Vectors,
pubmed-meshheading:16376852-Glutathione Transferase,
pubmed-meshheading:16376852-Ligands,
pubmed-meshheading:16376852-Lipid Metabolism,
pubmed-meshheading:16376852-Mass Spectrometry,
pubmed-meshheading:16376852-Mice,
pubmed-meshheading:16376852-Mice, Transgenic,
pubmed-meshheading:16376852-PPAR gamma,
pubmed-meshheading:16376852-Peroxisome Proliferator-Activated Receptors,
pubmed-meshheading:16376852-Plasmids,
pubmed-meshheading:16376852-Protein Isoforms,
pubmed-meshheading:16376852-Protein Structure, Tertiary,
pubmed-meshheading:16376852-RNA, Messenger,
pubmed-meshheading:16376852-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16376852-Time Factors,
pubmed-meshheading:16376852-cis-trans-Isomerases
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pubmed:year |
2006
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pubmed:articleTitle |
C/EBPalpha-dependent induction of glutathione S-transferase zeta/maleylacetoacetate isomerase (GSTzeta/MAAI) expression during the differentiation of mouse fibroblasts into adipocytes.
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pubmed:affiliation |
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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