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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-4-3
pubmed:abstractText
Inorganic arsenic has strong human carcinogenic potential, but the availability of an animal model to study toxicity is extremely limited. Here, we used the transgenic zebrafish line Tg(k18(2.9):RFP) as an animal model to study arsenite toxicity. This line was chosen because the red fluorescent protein (RFP) is expressed in stratified epithelia (including skin), due to the RFP reporter driven by the promoter of the zebrafish keratin 18 gene. We titrated doses of inorganic arsenite for zebrafish embryos and found that arsenite exposure at 50 microM for 120 h was suitable for mimicking a long-term, chronic effect. When embryos derived from Tg(k18(2.9):RFP) adults were treated with this arsenite dose and time of exposure, abnormal phenotypes were not noticeable under the light microscope. However, arsenic keratosis was visible in the epithelial cells under the fluorescent microscope. Morphological defects became more severe with increased dose and exposure duration, suggesting that the severity of skin lesions was dose- and time-dependent. Histochemical examination of keratosis after 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) staining showed that the epithelial cells overproliferated after treatment with arsenite. Therefore, this Tg(k18(2.9):RFP) zebrafish line is an excellent model for studying toxicity induced by inorganic arsenite and may have potential for studying other environmental pollutants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16376500-Animals, pubmed-meshheading:16376500-Animals, Genetically Modified, pubmed-meshheading:16376500-Arsenites, pubmed-meshheading:16376500-Cell Proliferation, pubmed-meshheading:16376500-Dose-Response Relationship, Drug, pubmed-meshheading:16376500-Embryo, Nonmammalian, pubmed-meshheading:16376500-Epithelial Cells, pubmed-meshheading:16376500-Keratin-18, pubmed-meshheading:16376500-Keratins, pubmed-meshheading:16376500-Keratosis, pubmed-meshheading:16376500-Linear Models, pubmed-meshheading:16376500-Luminescent Proteins, pubmed-meshheading:16376500-Microscopy, Fluorescence, pubmed-meshheading:16376500-Models, Animal, pubmed-meshheading:16376500-Promoter Regions, Genetic, pubmed-meshheading:16376500-Random Allocation, pubmed-meshheading:16376500-Skin, pubmed-meshheading:16376500-Teratogens, pubmed-meshheading:16376500-Toxicity Tests, pubmed-meshheading:16376500-Zebrafish, pubmed-meshheading:16376500-Zebrafish Proteins
pubmed:year
2006
pubmed:articleTitle
A keratin 18 transgenic zebrafish Tg(k18(2.9):RFP) treated with inorganic arsenite reveals visible overproliferation of epithelial cells.
pubmed:affiliation
Institute of Molecular and Cellular Biology, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't