Linked Life Data

16375885

Source:http://linkedlifedata.com/resource/pubmed/id/16375885

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-1-17
pubmed:abstractText
Vascular endothelial growth factor-A (VEGF-A) signaling directs both vasculogenesis and angiogenesis. However, the role of VEGF-A ligand signaling in the regulation of epithelial-mesenchymal interactions during early mouse lung morphogenesis remains incompletely characterized. Fetal liver kinase-1 (Flk-1) is a VEGF cognate receptor (VEGF-R2) expressed in the embryonic lung mesenchyme. VEGF-A, expressed in the epithelium, is a high affinity ligand for Flk-1. We have used both gain and loss of function approaches to investigate the role of this VEGF-A signaling pathway during lung morphogenesis. Herein, we demonstrate that exogenous VEGF 164, one of the 3 isoforms generated by alternative splicing of the Vegf-A gene, stimulates mouse embryonic lung branching morphogenesis in culture and increases the index of proliferation in both epithelium and mesenchyme. In addition, it induces differential gene and protein expression among several key lung morphogenetic genes, including up-regulation of BMP-4 and Sp-c expression as well as an increase in Flk-1-positive mesenchymal cells. Conversely, embryonic lung culture with an antisense oligodeoxynucleotide (ODN) to the Flk-1 receptor led to reduced epithelial branching, decreased epithelial and mesenchymal proliferation index as well as downregulating BMP-4 expression. These results demonstrate that the VEGF pathway is involved in driving epithelial to endothelial crosstalk in embryonic mouse lung morphogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
290
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-88
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16375885-Animals, pubmed-meshheading:16375885-Bone Morphogenetic Protein 4, pubmed-meshheading:16375885-Bone Morphogenetic Proteins, pubmed-meshheading:16375885-Cell Differentiation, pubmed-meshheading:16375885-Cell Proliferation, pubmed-meshheading:16375885-Endothelium, pubmed-meshheading:16375885-Epithelium, pubmed-meshheading:16375885-Gene Expression Regulation, Developmental, pubmed-meshheading:16375885-Lung, pubmed-meshheading:16375885-Mesoderm, pubmed-meshheading:16375885-Mice, pubmed-meshheading:16375885-Morphogenesis, pubmed-meshheading:16375885-Organ Culture Techniques, pubmed-meshheading:16375885-Peptides, pubmed-meshheading:16375885-Signal Transduction, pubmed-meshheading:16375885-Vascular Endothelial Growth Factor A, pubmed-meshheading:16375885-Vascular Endothelial Growth Factor Receptor-2
pubmed:year
2006
pubmed:articleTitle
VEGF-A signaling through Flk-1 is a critical facilitator of early embryonic lung epithelial to endothelial crosstalk and branching morphogenesis.
pubmed:affiliation
Developmental Biology Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatric Surgery, USC Keck School of Medicine, 4650 Sunset Blvd., Los Angeles, CA 90027, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural