Linked Life Data

16373352

Source:http://linkedlifedata.com/resource/pubmed/id/16373352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2006-2-27
pubmed:abstractText
Neurotransmitter release from presynaptic nerve terminals is restricted to specialized areas of the plasma membrane, so-called active zones. Active zones are characterized by a network of cytoplasmic scaffolding proteins involved in active zone generation and synaptic transmission. To analyze the modes of biogenesis of this cytomatrix, we asked how Bassoon and Piccolo, two prototypic active zone cytomatrix molecules, are delivered to nascent synapses. Although these proteins may be transported via vesicles, little is known about the importance of a vesicular pathway and about molecular determinants of cytomatrix molecule trafficking. We found that Bassoon and Piccolo co-localize with markers of the trans-Golgi network in cultured neurons. Impairing vesicle exit from the Golgi complex, either using brefeldin A, recombinant proteins, or a low temperature block, prevented transport of Bassoon out of the soma. Deleting a newly identified Golgi-binding region of Bassoon impaired subcellular targeting of recombinant Bassoon. Overexpressing this region to specifically block Golgi binding of the endogenous protein reduced the concentration of Bassoon at synapses. These results suggest that, during the period of bulk synaptogenesis, a primordial cytomatrix assembles in a trans-Golgi compartment. They further indicate that transport via Golgi-derived vesicles is essential for delivery of cytomatrix proteins to the synapse. Paradigmatically this establishes Golgi transit as an obligatory step for subcellular trafficking of distinct cytoplasmic scaffolding proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6038-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16373352-Animals, pubmed-meshheading:16373352-Biological Markers, pubmed-meshheading:16373352-Brefeldin A, pubmed-meshheading:16373352-Cells, Cultured, pubmed-meshheading:16373352-Cytoskeletal Proteins, pubmed-meshheading:16373352-Cytoskeleton, pubmed-meshheading:16373352-Golgi Apparatus, pubmed-meshheading:16373352-Nerve Tissue Proteins, pubmed-meshheading:16373352-Neurons, pubmed-meshheading:16373352-Neuropeptides, pubmed-meshheading:16373352-Protein Structure, Tertiary, pubmed-meshheading:16373352-Protein Synthesis Inhibitors, pubmed-meshheading:16373352-Protein Transport, pubmed-meshheading:16373352-Rats, pubmed-meshheading:16373352-Recombinant Fusion Proteins, pubmed-meshheading:16373352-Synapses, pubmed-meshheading:16373352-Synaptic Vesicles, pubmed-meshheading:16373352-trans-Golgi Network
pubmed:year
2006
pubmed:articleTitle
Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment.
pubmed:affiliation
Leibniz Institute for Neurobiology, Brenneckestrasse 6, D-39118 Magdeburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural