Linked Life Data

16368546

Source:http://linkedlifedata.com/resource/pubmed/id/16368546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2005-12-21
pubmed:abstractText
HSD-3.8 cDNA (accession number AF311312) encodes a human sperm component. A 0.7 kb fragment (HSD-0.7) containing three immunological epitopes of HSD-3.8 cDNA was prepared and expressed in E. coli. Immunization of female rats with the recombinant HSD-0.7 proteins induced infertility. A cDNA fragment encoding the C-terminal 144 amino acids of human G-protein beta l subunit (Gbeta1-C144) was screened by yeast two-hybrid, when HSD-0.7 segment was used as a bait. Recombinant His6-tagged-Gbeta1-C144 protein was expressed in E. coli BL21 and Anti-Gbeta1 serum was raised with purified Gbeta1-C144. HA-tagged HSD-0.7 and FLAG-tagged Gbeta1 plasmids were constructed and co-transfected into human embryonal kidney 293 cells. Two proteins were localized at superimposable sites in the cytoplasm, and they formed a complex when 500 micromol/L GDP existed. Overexpression of HSD-0.7 activated the G-protein-mediated extracellular signal-regulated kinases (ERK1/2); however, the truncated fragments of HSD-0.7, which lacked either TPR domain or P-loop, lost the ability to activate the ERK1/2 pathway. Further study revealed that the activation of ERK1/2 was protein kinase C (PKC) rather than Ras dependent. These results provide evidence that HSD-3.8 present in spermatocytes and sperm may participate in spermatogenesis and fertilization process by activating the PKC-dependent ERK1/2 signal transduction pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1093-4715
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1679-89
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16368546-Animals, pubmed-meshheading:16368546-Antigens, Surface, pubmed-meshheading:16368546-Blotting, Western, pubmed-meshheading:16368546-COS Cells, pubmed-meshheading:16368546-Cell Line, pubmed-meshheading:16368546-Cercopithecus aethiops, pubmed-meshheading:16368546-Cytoplasm, pubmed-meshheading:16368546-DNA, Complementary, pubmed-meshheading:16368546-Escherichia coli, pubmed-meshheading:16368546-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:16368546-Female, pubmed-meshheading:16368546-GTP-Binding Protein beta Subunits, pubmed-meshheading:16368546-GTP-Binding Proteins, pubmed-meshheading:16368546-Humans, pubmed-meshheading:16368546-Immunoprecipitation, pubmed-meshheading:16368546-MAP Kinase Signaling System, pubmed-meshheading:16368546-Male, pubmed-meshheading:16368546-Microscopy, Fluorescence, pubmed-meshheading:16368546-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:16368546-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:16368546-Ovary, pubmed-meshheading:16368546-Plasmids, pubmed-meshheading:16368546-Protein Kinase C, pubmed-meshheading:16368546-Protein Structure, Tertiary, pubmed-meshheading:16368546-Recombinant Proteins, pubmed-meshheading:16368546-Signal Transduction, pubmed-meshheading:16368546-Spermatogenesis, pubmed-meshheading:16368546-Spermatozoa, pubmed-meshheading:16368546-Testis, pubmed-meshheading:16368546-Tissue Distribution, pubmed-meshheading:16368546-Transfection, pubmed-meshheading:16368546-Two-Hybrid System Techniques
pubmed:year
2006
pubmed:articleTitle
A sperm component, HSD-3.8 (SPAG1), interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK).
pubmed:affiliation
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing, 100005, People Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't