Linked Life Data

16365428

Source:http://linkedlifedata.com/resource/pubmed/id/16365428

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-12-20
pubmed:abstractText
Ig-proteolipid protein 1 (Ig-PLP1) is an Ig chimera expressing the encephalitogenic PLP1 peptide corresponding to amino acid residues 139-151 of PLP. Newborn mice given Ig-PLP1 in saline on the day of birth and challenged 7 wk later with PLP1 peptide in CFA develop an organ-specific neonatal immunity that confers resistance against experimental allergic encephalomyelitis. The T cell responses in these animals are comprised of Th2 cells in the lymph node and anergic Th1 lymphocytes in the spleen. Intriguingly, the anergic splenic T cells, although nonproliferative and unable to produce IFN-gamma or IL-4, secrete significant amounts of IL-2. Studies were performed to determine whether the two populations display any structural differences in the TCR H chain variable region that could contribute to the differential affinity and retention in different organs. Responsive Th2 lymph node T cells and anergic splenic lymphocytes were immortalized, and the structures of their TCR Vbeta were determined. The results show that Vbeta and Jbeta usage was random, but the CDR3 regions of the lymph node cells had a conserved Gly-Gly motif. Analysis of TCR affinity/avidity correlated the Gly-Gly motif with lower affinity and retention of the Th2 cells in the lymph node. Also, it is suggested that a higher TCR affinity may be a contributing factor for the development of the neonatal Th1 response in the spleen.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
176
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
357-64
pubmed:dateRevised
2008-7-9
pubmed:meshHeading
pubmed-meshheading:16365428-Amino Acid Motifs, pubmed-meshheading:16365428-Animals, pubmed-meshheading:16365428-Animals, Newborn, pubmed-meshheading:16365428-Base Sequence, pubmed-meshheading:16365428-Clonal Anergy, pubmed-meshheading:16365428-Complementarity Determining Regions, pubmed-meshheading:16365428-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16365428-Flow Cytometry, pubmed-meshheading:16365428-Hybridomas, pubmed-meshheading:16365428-Lymph Nodes, pubmed-meshheading:16365428-Mice, pubmed-meshheading:16365428-Molecular Sequence Data, pubmed-meshheading:16365428-Myelin Proteolipid Protein, pubmed-meshheading:16365428-Nerve Tissue Proteins, pubmed-meshheading:16365428-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:16365428-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16365428-Spleen, pubmed-meshheading:16365428-T-Lymphocytes
pubmed:year
2006
pubmed:articleTitle
neonatally primed lymph node, but not splenic T cells, display a Gly-Gly motif within the TCR beta-chain complementarity-determining region 3 that controls affinity and may affect lymphoid organ retention.
pubmed:affiliation
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural