rdf:type |
|
lifeskim:mentions |
umls-concept:C0000843,
umls-concept:C0038435,
umls-concept:C0162741,
umls-concept:C0220905,
umls-concept:C0311417,
umls-concept:C1335864,
umls-concept:C1419697,
umls-concept:C1514562,
umls-concept:C1521802,
umls-concept:C1710082,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2587213
|
pubmed:issue |
8
|
pubmed:dateCreated |
2006-2-20
|
pubmed:abstractText |
ABI1 and ABI2 encode PP2C-type protein phosphatases and are thought to negatively regulate many aspects of abscisic acid (ABA) signaling, including stomatal closure in Arabidopsis. In contrast, SRK2E/OST1/SnRK2.6 encodes an Arabidopsis SnRK2 protein kinase and acts as a positive regulator in the ABA-induced stomatal closure. SRK2E/OST1 is activated by osmotic stress as well as by ABA, but the independence of the two activation processes has not yet been determined. Additionally, interaction between SRK2E/OST1 and PP2C-type phosphatases (ABI1 and ABI2) is not understood. In the present study, we demonstrated that the abi1-1 mutation, but not the abi2-1 mutation, strongly inhibited ABA-dependent SRK2E/OST1 activation. In contrast, osmotic stress activated SRK2E/OST1 even in abi1-1 and aba2-1 plants. The C-terminal regulatory domain of SRK2E/OST1 was required for its activation by both ABA and osmotic stress in Arabidopsis. The C-terminal domain was functionally divided into Domains I and II. Domain II was required only for the ABA-dependent activation of SRK2E/OST1, whereas Domain I was responsible for the ABA-independent activation. Full-length SRK2E/OST1 completely complemented the wilty phenotype of the srk2e mutant, but SRK2E/OST1 lacking Domain II did not. Domain II interacted with the ABI1 protein in a yeast two-hybrid assay. Our results suggested that the direct interaction between SRK2E/OST1 and ABI1 through Domain II plays a critical role in the control of stomatal closure.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ABI1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Abscisic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrate Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/OST1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Growth Regulators,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0021-9258
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
24
|
pubmed:volume |
281
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
5310-8
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:16365038-Abscisic Acid,
pubmed-meshheading:16365038-Amino Acid Sequence,
pubmed-meshheading:16365038-Arabidopsis,
pubmed-meshheading:16365038-Arabidopsis Proteins,
pubmed-meshheading:16365038-Gene Expression Regulation, Plant,
pubmed-meshheading:16365038-Genes, Plant,
pubmed-meshheading:16365038-Green Fluorescent Proteins,
pubmed-meshheading:16365038-Models, Biological,
pubmed-meshheading:16365038-Molecular Sequence Data,
pubmed-meshheading:16365038-Mutation,
pubmed-meshheading:16365038-Nitrate Reductase,
pubmed-meshheading:16365038-Osmosis,
pubmed-meshheading:16365038-Phenotype,
pubmed-meshheading:16365038-Phosphoprotein Phosphatases,
pubmed-meshheading:16365038-Plant Epidermis,
pubmed-meshheading:16365038-Plant Growth Regulators,
pubmed-meshheading:16365038-Plants, Genetically Modified,
pubmed-meshheading:16365038-Protein Binding,
pubmed-meshheading:16365038-Protein Kinases,
pubmed-meshheading:16365038-Protein Structure, Tertiary,
pubmed-meshheading:16365038-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16365038-Sequence Homology, Amino Acid,
pubmed-meshheading:16365038-Signal Transduction,
pubmed-meshheading:16365038-Two-Hybrid System Techniques
|
pubmed:year |
2006
|
pubmed:articleTitle |
The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis.
|
pubmed:affiliation |
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|