Linked Life Data

16349657

Source:http://linkedlifedata.com/resource/pubmed/id/16349657

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-1-31
pubmed:abstractText
The growth of Pasteurella haemolytica strain H44L was studied under aerobic conditions in a medium of acid-hydrolyzed casein, supplementary cysteine, inorganic salts, vitamins, and a carbon source. The concentration of casein hydrolysate necessary for optimal growth was 1.5 or 2.0%, depending upon the carbon source employed. Essential vitamins were calcium pantothenate, nicotinamide, and thiamine. Concentrations as low as 0.01 mug/ml of thiamine monophosphate or thiamine pyrophosphate supported maximal growth, but thiamine hydrochloride or thiamine nitrate were active only at the unusually high levels of 10 to 20 mug/ml. The best carbon sources were d-galactose or sucrose. Maximal growth resulted from an inoculum containing fewer than 10 cells per milliliter of medium. Cellular yields averaged 6 x 10 to 7 x 10 cells per milliliter for the test organism and five other strains of P. haemolytica isolated from cases of bovine respiratory diseases.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
May
pubmed:issn
0003-6919
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
426-31
pubmed:dateRevised
2009-11-18
pubmed:year
1965
pubmed:articleTitle
Cultivation of Pasteurella haemolytica in a Casein Hydrolysate Medium.
pubmed:affiliation
National Animal Disease Laboratory, Animal Disease and Parasite Research Division, U.S. Department of Agriculture, Ames, Iowa.
pubmed:publicationType
Journal Article