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pubmed-article:16348341pubmed:abstractTextA number of Pseudomonas species have been identified which accumulate a polyhydroxyalkanoate containing mainly 3-hydroxydecanoate monomers from sodium gluconate as the sole carbon source. One of these, Pseudomonas sp. strain NCIMB 40135, was further investigated and shown to accumulate such a polyhydroxyalkanoate from a wide range of carbon sources (C(2) to C(6)); however, when supplied with octanoic acid it produced a polyhydroxyalkanoate containing mainly 3-hydroxyoctanoate monomers. Polymer synthesis occurred in batch culture after cessation of growth due to exhaustion of nitrogen. In continuous culture under nitrogen limitation up to 16.9% (wt/wt) polyhydroxyalkanoate was synthesized from glucose as the carbon source. The monomer units are mainly of the R-(-) configuration. Nuclear magnetic resonance studies confirmed the composition of the polymer. Differential scanning calorimetry suggested that the solvent-extracted polymer contained a significant proportion of crystalline material. The weight-average molecular weight of the polymer from glucose-grown cells was 143,000.lld:pubmed
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pubmed-article:16348341pubmed:monthNovlld:pubmed
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pubmed-article:16348341pubmed:authorpubmed-author:DawesE AEAlld:pubmed
pubmed-article:16348341pubmed:authorpubmed-author:AndersonA JAJlld:pubmed
pubmed-article:16348341pubmed:authorpubmed-author:EwingD FDFlld:pubmed
pubmed-article:16348341pubmed:authorpubmed-author:HaywoodG WGWlld:pubmed
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pubmed-article:16348341pubmed:volume56lld:pubmed
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pubmed-article:16348341pubmed:pagination3354-9lld:pubmed
pubmed-article:16348341pubmed:dateRevised2010-9-20lld:pubmed
pubmed-article:16348341pubmed:year1990lld:pubmed
pubmed-article:16348341pubmed:articleTitleAccumulation of a Polyhydroxyalkanoate Containing Primarily 3-Hydroxydecanoate from Simple Carbohydrate Substrates by Pseudomonas sp. Strain NCIMB 40135.lld:pubmed
pubmed-article:16348341pubmed:affiliationDepartments of Applied Biology and Chemistry, University of Hull, Hull HU6 7RX, United Kingdom.lld:pubmed
pubmed-article:16348341pubmed:publicationTypeJournal Articlelld:pubmed
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