Source:http://linkedlifedata.com/resource/pubmed/id/16348341
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2010-6-25
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pubmed:abstractText |
A number of Pseudomonas species have been identified which accumulate a polyhydroxyalkanoate containing mainly 3-hydroxydecanoate monomers from sodium gluconate as the sole carbon source. One of these, Pseudomonas sp. strain NCIMB 40135, was further investigated and shown to accumulate such a polyhydroxyalkanoate from a wide range of carbon sources (C(2) to C(6)); however, when supplied with octanoic acid it produced a polyhydroxyalkanoate containing mainly 3-hydroxyoctanoate monomers. Polymer synthesis occurred in batch culture after cessation of growth due to exhaustion of nitrogen. In continuous culture under nitrogen limitation up to 16.9% (wt/wt) polyhydroxyalkanoate was synthesized from glucose as the carbon source. The monomer units are mainly of the R-(-) configuration. Nuclear magnetic resonance studies confirmed the composition of the polymer. Differential scanning calorimetry suggested that the solvent-extracted polymer contained a significant proportion of crystalline material. The weight-average molecular weight of the polymer from glucose-grown cells was 143,000.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-13747777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-13878063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-16347708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-16347790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-2125185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-2506811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-2518731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-4594739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-5821736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348341-6388497
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0099-2240
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3354-9
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pubmed:dateRevised |
2010-9-20
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pubmed:year |
1990
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pubmed:articleTitle |
Accumulation of a Polyhydroxyalkanoate Containing Primarily 3-Hydroxydecanoate from Simple Carbohydrate Substrates by Pseudomonas sp. Strain NCIMB 40135.
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pubmed:affiliation |
Departments of Applied Biology and Chemistry, University of Hull, Hull HU6 7RX, United Kingdom.
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pubmed:publicationType |
Journal Article
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