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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-6-25
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pubmed:abstractText |
Twenty different bacterial isolates obtained from a mercury-contaminated site in Oak Ridge, Tenn., were grown on plate count agar amended with 25 mug of Hg or 3 mug of CH(3)-Hg (R-Hg) per ml. The total cellular RNA was extracted from each isolate by an acid-guanidine-thiocyanate-phenol-chloroform method. The transcripts of merA and merB were detected and quantitated by Northern (RNA) hybridization. A qualitative assay of mercuric reductase was used to confirm the enzyme activity. Low temperature (4 degrees C) with the presence of Hg (25 mug/ml) significantly increased the net merA transcripts of mid-log-phase cells of six environmental isolates. The net merA transcript production by 18 of the isolates increased when they were grown on 50% plate count broth with 15 mug of Hg per ml, but only 8 isolates showed increased production of merB transcripts. The MICs of Hg and R-Hg for 10 methyl mercury-resistant isolates ranged from 45 to 110 mug of Hg and 0.6 to 4.5 mug of R-Hg per ml. R-Hg was able to induce the expression of merB in 70% of methyl mercury-resistant strains.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-16347844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-16348130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-17811660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2310185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2444159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2492496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2513777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2547336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2581935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2629842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2648565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2827958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-2910495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3033633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3060006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3063210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3085085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-350872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3521892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3535655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3753001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-3894571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-4005712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-4581286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-4632313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-6277900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-6286594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-6340733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-6436233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-914774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16348333-9382
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3266-72
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pubmed:dateRevised |
2010-9-20
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pubmed:year |
1990
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pubmed:articleTitle |
Effects of Hg, CH(3)-Hg, and Temperature on the Expression of Mercury Resistance Genes in Environmental Bacteria.
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pubmed:affiliation |
Program in Social Ecology, University of California, Irvine, California 92717.
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pubmed:publicationType |
Journal Article
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